|
Significant 16S sequence loss while merging w/ DADA2, various truncation settings used
|
|
2
|
54
|
November 5, 2025
|
|
Analysis of 16S data from different regions using q2-fragment-insertion or other substitution
|
|
3
|
36
|
August 10, 2025
|
|
Is it necessary to separate ASVs classified at genus level into their respective sequences in ANCOMBC2 or other differential abundance analysis?
|
|
2
|
78
|
July 8, 2025
|
|
Reconciling 16s Databases in a Single Pipeline
|
|
5
|
188
|
August 28, 2024
|
|
Specificity and Sensitivity at Different Abundances?
|
|
3
|
51
|
August 24, 2024
|
|
Does QIIME2 support V1-V9 taxonomic classification?
|
|
2
|
444
|
August 30, 2023
|
|
Questions about 16S data from Novogene (UK)
|
|
29
|
2530
|
September 20, 2023
|
|
Is it best to work on abundance tables of ASVs or taxa?
|
|
5
|
1471
|
May 23, 2022
|
|
Losing a LOT of representative sequences after denoising
|
|
6
|
974
|
December 24, 2021
|
|
Importing Multiplexed Paired End Sequence With Adaptors --> Demultiplexed Sequences
|
|
4
|
1260
|
December 24, 2021
|
|
Primer naming inconsistencies?
|
|
2
|
1030
|
September 29, 2021
|
|
Taxonomic assignment at the species level
|
|
2
|
1279
|
August 13, 2021
|
|
Questions about V3–V4 primers for 16S rRNA amplicon sequencing, and calculating overlap
|
|
3
|
21309
|
August 21, 2021
|
|
Methods for integrating 16S and RNAseq data
|
|
2
|
562
|
April 1, 2021
|
|
16S V3-V4 length : what to do with the sequences of shorter than expected length after DADA2?
|
|
10
|
5832
|
December 8, 2020
|
|
MiSeq output quality distribution.
|
|
0
|
926
|
September 24, 2020
|
|
Can we match 16s species to metagenomics species?
|
|
0
|
507
|
January 21, 2020
|
|
Normalization for microbiome 16s sequence analysis
|
|
9
|
5492
|
June 27, 2019
|
|
16s circular phylogenetic tree
|
|
1
|
753
|
January 16, 2019
|
|
How Do I Recover 18s Reads after Filtering in Qiime 2?
|
|
5
|
1519
|
June 19, 2018
|
|
16S copy number normalization?
|
|
3
|
3610
|
February 16, 2018
|
|
Downstream Analysis of 16S Illumina Sequence Files
|
|
2
|
1114
|
February 6, 2018
|
|
Extract reads - SILVA 128 issue
|
|
2
|
1446
|
January 23, 2018
|
|
Need/How/when to separate 16S and ITS on the same run (MiSeq)
|
|
4
|
1216
|
November 9, 2017
|