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DADA2 denoising taking long time
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1
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4
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November 27, 2025
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Error was encountered while running DADA2 in R (return code 1)
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4
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18
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November 26, 2025
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DADA2 priors trimming
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1
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10
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November 18, 2025
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priors in qiime2-dada2
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4
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238
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November 18, 2025
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QIIME2 non-chimeric reads
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3
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41
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November 10, 2025
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DADA2 -- denoise
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2
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50
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November 7, 2025
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Significant 16S sequence loss while merging w/ DADA2, various truncation settings used
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2
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77
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November 5, 2025
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DADA2 Fails on AVITI Data
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7
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77
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October 29, 2025
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qiime dada2 denoise-paired is unresponsive
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2
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40
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October 27, 2025
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Interpreting KEGG vs MetaCyc pathways in 16S (PICRUSt2) functional prediction
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1
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35
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October 18, 2025
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Observed ASV richness drops after adding 3rd MiSeq library — why do I lose ~100 species when I merge libraries?
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4
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23
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November 16, 2025
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ITS denoising truncation
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2
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30
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November 15, 2025
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Need help choosing truncation lengths for DADA2 and interpreting the demultiplexed sequence count summary.
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2
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35
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November 13, 2025
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Converting 16S V3–V4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?)
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3
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75
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October 9, 2025
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Chimera removal dilemma in 16S V3–V4: necessary when using Kraken2/Bracken?
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8
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85
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September 26, 2025
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Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis?
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4
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98
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September 18, 2025
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Trained classifier output not found
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7
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78
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October 13, 2025
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16S CCS without primers: how to skip --p-front/--p-adapter in denoise-ccs?
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4
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65
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September 26, 2025
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Percentage of reads discarded after running DADA2 on NextSeq data
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1
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48
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August 25, 2025
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Effect of primer removal on chimera detection in DADA2
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11
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156
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September 26, 2025
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Shall I need to remove ASV with prevelance in one sample?
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4
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60
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August 8, 2025
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Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)?
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4
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71
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July 22, 2025
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Less percentage of reads merged in qiime-DADA2 even with enough overlapping length present.
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7
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76
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August 11, 2025
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dada2 error: rcpp/SparseArray
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2
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33
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August 10, 2025
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PICRUST2 Input Data setting
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1
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58
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July 8, 2025
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Please help interpreting demux data
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2
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62
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July 18, 2025
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Minimum length of read in ASV (DADA2)
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6
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112
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July 9, 2025
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Shotgun sequencing and Trimming
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2
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38
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June 3, 2025
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Understanding min sequence length of F and R/ quality scores and how it is impacting dada2 downstream
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15
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133
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July 4, 2025
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Denoise pair returning DataFrame' object has no attribute 'map' error
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6
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71
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July 4, 2025
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