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Too many elements for DADA2 - denoise in batches?
|
|
2
|
67
|
February 26, 2026
|
|
Inadequate overlap for V3-V4 (paired-end) due to quality drop
|
|
0
|
15
|
February 26, 2026
|
|
denoise failure
|
|
5
|
37
|
February 24, 2026
|
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Integrating V3-V4 and V4 16S datasets for large-scale meta-analysis: Is Greengenes2 the optimal choice for cross-region alignment?
|
|
0
|
45
|
January 29, 2026
|
|
Huge difference of DADA2 output with and without removing primers
|
|
5
|
74
|
February 27, 2026
|
|
Advice in best practice for handling multiple sequencing runs and subset analyses in QIIME 2 (DADA2)
|
|
2
|
33
|
January 16, 2026
|
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pair ended demultiplexed qza file causing strange error.
|
|
2
|
43
|
January 29, 2026
|
|
High read loss due to artifacts and chimera after denoising 16S V3-V4 paired-end reads
|
|
2
|
52
|
January 17, 2026
|
|
Denoising low quality reverse reads in paired-end sequencing reads
|
|
5
|
67
|
January 3, 2026
|
|
Issue with UNITE classifier for ITS1
|
|
2
|
49
|
January 2, 2026
|
|
Error was encountered while running DADA2 in R (return code 1)
|
|
9
|
92
|
January 5, 2026
|
|
DADA2 denoising taking long time
|
|
7
|
76
|
January 1, 2026
|
|
Questions about performing quality control with dada2 and generating a feature table
|
|
3
|
37
|
December 30, 2025
|
|
DADA2 priors trimming
|
|
1
|
29
|
November 18, 2025
|
|
priors in qiime2-dada2
|
|
4
|
250
|
November 18, 2025
|
|
QIIME2 non-chimeric reads
|
|
4
|
66
|
December 11, 2025
|
|
DADA2 -- denoise
|
|
2
|
62
|
November 7, 2025
|
|
Significant 16S sequence loss while merging w/ DADA2, various truncation settings used
|
|
3
|
113
|
December 6, 2025
|
|
DADA2 Fails on AVITI Data
|
|
8
|
133
|
November 29, 2025
|
|
qiime dada2 denoise-paired is unresponsive
|
|
3
|
58
|
November 27, 2025
|
|
Interpreting KEGG vs MetaCyc pathways in 16S (PICRUSt2) functional prediction
|
|
1
|
85
|
October 18, 2025
|
|
Observed ASV richness drops after adding 3rd MiSeq library — why do I lose ~100 species when I merge libraries?
|
|
4
|
34
|
November 16, 2025
|
|
ITS denoising truncation
|
|
2
|
42
|
November 15, 2025
|
|
Need help choosing truncation lengths for DADA2 and interpreting the demultiplexed sequence count summary.
|
|
2
|
42
|
November 13, 2025
|
|
Converting 16S V3–V4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?)
|
|
3
|
89
|
October 9, 2025
|
|
Chimera removal dilemma in 16S V3–V4: necessary when using Kraken2/Bracken?
|
|
8
|
133
|
September 26, 2025
|
|
Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis?
|
|
4
|
142
|
September 18, 2025
|
|
Trained classifier output not found
|
|
7
|
107
|
October 13, 2025
|
|
16S CCS without primers: how to skip --p-front/--p-adapter in denoise-ccs?
|
|
4
|
86
|
September 26, 2025
|
|
Percentage of reads discarded after running DADA2 on NextSeq data
|
|
1
|
64
|
August 25, 2025
|
