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Observed ASV richness drops after adding 3rd MiSeq library — why do I lose ~100 species when I merge libraries?
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3
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7
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October 16, 2025
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ITS denoising truncation
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1
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9
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October 15, 2025
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Need help choosing truncation lengths for DADA2 and interpreting the demultiplexed sequence count summary.
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1
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13
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October 13, 2025
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Converting 16S V3–V4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?)
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3
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42
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October 9, 2025
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Chimera removal dilemma in 16S V3–V4: necessary when using Kraken2/Bracken?
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8
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64
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September 26, 2025
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Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis?
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4
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74
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September 18, 2025
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Trained classifier output not found
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7
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49
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October 13, 2025
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16S CCS without primers: how to skip --p-front/--p-adapter in denoise-ccs?
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4
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38
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September 26, 2025
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Percentage of reads discarded after running DADA2 on NextSeq data
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1
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42
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August 25, 2025
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Effect of primer removal on chimera detection in DADA2
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11
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130
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September 26, 2025
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Shall I need to remove ASV with prevelance in one sample?
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4
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39
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August 8, 2025
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Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)?
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4
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51
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July 22, 2025
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Less percentage of reads merged in qiime-DADA2 even with enough overlapping length present.
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7
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58
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August 11, 2025
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dada2 error: rcpp/SparseArray
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2
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22
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August 10, 2025
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PICRUST2 Input Data setting
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1
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42
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July 8, 2025
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Please help interpreting demux data
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2
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41
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July 18, 2025
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Minimum length of read in ASV (DADA2)
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6
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87
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July 9, 2025
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Shotgun sequencing and Trimming
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2
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26
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June 3, 2025
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Understanding min sequence length of F and R/ quality scores and how it is impacting dada2 downstream
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15
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116
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July 4, 2025
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Denoise pair returning DataFrame' object has no attribute 'map' error
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6
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57
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July 4, 2025
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dada2-paired giving return code 1
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7
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42
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June 28, 2025
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DADA2 fails silently on SLURM cluster (QIIME2 2024.10) — No .qza outputs, no error messages
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2
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22
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June 23, 2025
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Error with DADA2 with sequences of different runs.
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4
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52
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June 21, 2025
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Can trimming and dada 2
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3
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49
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May 19, 2025
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Low percentage of non-quimeric reads after dada2
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4
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47
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June 14, 2025
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Alpha diversity with filtered raw data or unfiltered raw data
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1
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65
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April 24, 2025
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Losing a lot of reads during merging
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4
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81
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May 19, 2025
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QIIME 2 Meta-Analysis of Rhizosphere Microbiome with Mixed V3-V4/V4 Data: Best Practices for Downstream Analysis
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14
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350
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May 18, 2025
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Questions about the running time of qiime dada2 denoise-ccs
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4
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73
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May 13, 2025
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trunc length setting in dada2
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2
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14
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May 12, 2025
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