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pair ended demultiplexed qza file causing strange error.
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1
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15
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December 28, 2025
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High read loss due to artifacts and chimera after denoising 16S V3-V4 paired-end reads
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1
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27
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December 17, 2025
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Denoising low quality reverse reads in paired-end sequencing reads
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5
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49
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January 3, 2026
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Issue with UNITE classifier for ITS1
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2
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36
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January 2, 2026
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Error was encountered while running DADA2 in R (return code 1)
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9
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73
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January 5, 2026
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DADA2 denoising taking long time
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7
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43
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January 1, 2026
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Questions about performing quality control with dada2 and generating a feature table
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3
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28
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December 30, 2025
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DADA2 priors trimming
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1
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14
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November 18, 2025
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priors in qiime2-dada2
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4
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247
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November 18, 2025
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QIIME2 non-chimeric reads
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4
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55
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December 11, 2025
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DADA2 -- denoise
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2
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57
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November 7, 2025
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Significant 16S sequence loss while merging w/ DADA2, various truncation settings used
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3
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92
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December 6, 2025
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DADA2 Fails on AVITI Data
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8
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101
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November 29, 2025
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qiime dada2 denoise-paired is unresponsive
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3
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50
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November 27, 2025
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Interpreting KEGG vs MetaCyc pathways in 16S (PICRUSt2) functional prediction
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1
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47
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October 18, 2025
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Observed ASV richness drops after adding 3rd MiSeq library — why do I lose ~100 species when I merge libraries?
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4
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24
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November 16, 2025
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ITS denoising truncation
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2
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40
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November 15, 2025
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Need help choosing truncation lengths for DADA2 and interpreting the demultiplexed sequence count summary.
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2
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39
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November 13, 2025
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Converting 16S V3–V4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?)
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3
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82
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October 9, 2025
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Chimera removal dilemma in 16S V3–V4: necessary when using Kraken2/Bracken?
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8
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104
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September 26, 2025
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Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis?
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4
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116
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September 18, 2025
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Trained classifier output not found
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7
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93
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October 13, 2025
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16S CCS without primers: how to skip --p-front/--p-adapter in denoise-ccs?
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4
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74
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September 26, 2025
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Percentage of reads discarded after running DADA2 on NextSeq data
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1
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55
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August 25, 2025
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Effect of primer removal on chimera detection in DADA2
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11
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171
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September 26, 2025
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Shall I need to remove ASV with prevelance in one sample?
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4
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71
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August 8, 2025
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Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)?
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4
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105
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July 22, 2025
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Less percentage of reads merged in qiime-DADA2 even with enough overlapping length present.
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7
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89
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August 11, 2025
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dada2 error: rcpp/SparseArray
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2
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38
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August 10, 2025
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PICRUST2 Input Data setting
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1
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66
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July 8, 2025
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