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Integrating V3-V4 and V4 16S datasets for large-scale meta-analysis: Is Greengenes2 the optimal choice for cross-region alignment?
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1
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62
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March 6, 2026
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Too many elements for DADA2 - denoise in batches?
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6
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105
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March 4, 2026
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Analysis with DADA2... Merge Read Length
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1
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24
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March 4, 2026
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Inadequate overlap for V3-V4 (paired-end) due to quality drop
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3
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47
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March 3, 2026
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denoise failure
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5
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50
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February 24, 2026
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Huge difference of DADA2 output with and without removing primers
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5
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80
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February 27, 2026
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Advice in best practice for handling multiple sequencing runs and subset analyses in QIIME 2 (DADA2)
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2
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36
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January 16, 2026
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pair ended demultiplexed qza file causing strange error.
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2
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46
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January 29, 2026
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High read loss due to artifacts and chimera after denoising 16S V3-V4 paired-end reads
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2
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52
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January 17, 2026
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Denoising low quality reverse reads in paired-end sequencing reads
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5
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67
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January 3, 2026
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Issue with UNITE classifier for ITS1
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2
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49
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January 2, 2026
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Error was encountered while running DADA2 in R (return code 1)
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9
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92
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January 5, 2026
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DADA2 denoising taking long time
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7
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81
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January 1, 2026
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Questions about performing quality control with dada2 and generating a feature table
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3
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38
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December 30, 2025
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DADA2 priors trimming
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1
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30
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November 18, 2025
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priors in qiime2-dada2
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4
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251
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November 18, 2025
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QIIME2 non-chimeric reads
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4
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66
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December 11, 2025
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DADA2 -- denoise
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2
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62
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November 7, 2025
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Significant 16S sequence loss while merging w/ DADA2, various truncation settings used
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3
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114
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December 6, 2025
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DADA2 Fails on AVITI Data
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8
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146
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November 29, 2025
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qiime dada2 denoise-paired is unresponsive
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3
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58
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November 27, 2025
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Interpreting KEGG vs MetaCyc pathways in 16S (PICRUSt2) functional prediction
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1
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90
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October 18, 2025
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Observed ASV richness drops after adding 3rd MiSeq library — why do I lose ~100 species when I merge libraries?
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4
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34
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November 16, 2025
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ITS denoising truncation
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2
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46
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November 15, 2025
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Need help choosing truncation lengths for DADA2 and interpreting the demultiplexed sequence count summary.
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2
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47
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November 13, 2025
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Converting 16S V3–V4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?)
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3
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92
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October 9, 2025
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Chimera removal dilemma in 16S V3–V4: necessary when using Kraken2/Bracken?
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8
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138
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September 26, 2025
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Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis?
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4
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145
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September 18, 2025
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Trained classifier output not found
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7
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111
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October 13, 2025
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16S CCS without primers: how to skip --p-front/--p-adapter in denoise-ccs?
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4
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88
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September 26, 2025
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