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High read loss due to artifacts and chimera after denoising 16S V3-V4 paired-end reads
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1
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12
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December 17, 2025
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Denoising low quality reverse reads in paired-end sequencing reads
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4
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39
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December 3, 2025
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Issue with UNITE classifier for ITS1
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1
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27
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December 2, 2025
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Error was encountered while running DADA2 in R (return code 1)
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8
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56
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December 1, 2025
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DADA2 denoising taking long time
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6
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34
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December 1, 2025
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Questions about performing quality control with dada2 and generating a feature table
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2
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22
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November 29, 2025
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DADA2 priors trimming
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1
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11
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November 18, 2025
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priors in qiime2-dada2
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4
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244
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November 18, 2025
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QIIME2 non-chimeric reads
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4
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47
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December 11, 2025
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DADA2 -- denoise
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2
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54
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November 7, 2025
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Significant 16S sequence loss while merging w/ DADA2, various truncation settings used
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3
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83
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December 6, 2025
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DADA2 Fails on AVITI Data
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8
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89
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November 29, 2025
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qiime dada2 denoise-paired is unresponsive
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3
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46
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November 27, 2025
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Interpreting KEGG vs MetaCyc pathways in 16S (PICRUSt2) functional prediction
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1
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42
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October 18, 2025
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Observed ASV richness drops after adding 3rd MiSeq library — why do I lose ~100 species when I merge libraries?
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4
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23
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November 16, 2025
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ITS denoising truncation
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2
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34
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November 15, 2025
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Need help choosing truncation lengths for DADA2 and interpreting the demultiplexed sequence count summary.
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2
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37
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November 13, 2025
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Converting 16S V3–V4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?)
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3
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78
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October 9, 2025
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Chimera removal dilemma in 16S V3–V4: necessary when using Kraken2/Bracken?
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8
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97
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September 26, 2025
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Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis?
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4
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102
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September 18, 2025
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Trained classifier output not found
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7
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84
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October 13, 2025
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16S CCS without primers: how to skip --p-front/--p-adapter in denoise-ccs?
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4
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68
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September 26, 2025
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Percentage of reads discarded after running DADA2 on NextSeq data
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1
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51
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August 25, 2025
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Effect of primer removal on chimera detection in DADA2
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11
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166
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September 26, 2025
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Shall I need to remove ASV with prevelance in one sample?
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4
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67
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August 8, 2025
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Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)?
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4
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95
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July 22, 2025
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Less percentage of reads merged in qiime-DADA2 even with enough overlapping length present.
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7
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81
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August 11, 2025
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dada2 error: rcpp/SparseArray
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2
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34
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August 10, 2025
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PICRUST2 Input Data setting
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1
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60
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July 8, 2025
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Please help interpreting demux data
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2
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69
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July 18, 2025
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