Latest General Discussion topics

Topic	Replies	Views	Activity
Frequent Questions and "Best of the QIIME 2 Forum" best-of-the-forum The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…	2	7774	February 27, 2020
About the General Discussion category	1	981	March 13, 2023
Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)? dada2	1	3	July 18, 2025
Recommended Step for Merging Metagenomics Datasets merging , merge	1	21	July 16, 2025
Quality control for shotgun metagenomics quality-control	5	526	July 17, 2025
Merging qiime2 sample metadata with exterior taxonomic classifications taxonomy , feature-table	1	15	July 13, 2025
PR2: Division vs. Phylum	1	20	July 11, 2025
How to account for uneven sampling taxonomy	1	29	July 10, 2025
Rarefaction vs Rarefying and when to use them in your analysis pipeline q2-boots	14	215	July 9, 2025
Is it necessary to separate ASVs classified at genus level into their respective sequences in ANCOMBC2 or other differential abundance analysis? 16s , ancom-bc	2	35	July 8, 2025
Transforming Alpha Diversity Metrics for Mixed Effects Modeling	2	23	July 8, 2025
Best workflow for samples with high off-target (host) amplification	7	54	July 8, 2025
Greengenes2 vs. SILVA queued , silva , greengenes2	0	47	July 3, 2025
High percentage of unassigned reads (metagenomics)	6	33	July 3, 2025
SILVA trained version for V3-V4 amplicon using 341F/785R primers	3	33	July 2, 2025
Looking for Sensible Qiime2 CLI Aliases q2cli	1	32	July 1, 2025
How to simplify the formula gini index in QIIME2? For example write in a R script. diversity , alpha	2	45	November 1, 2024
Looking for Comprehensive Qiime2 Documentation	7	124	June 23, 2025
Request for Pre-trained SILVA V1–V3 Naive Bayes Classifier feature-classifier	2	35	June 18, 2025
classify-sklearn vs classify-consensus-vsearch - classification depth taxonomy	2	47	June 13, 2025
Shotgun sequencing of Fungi - UNITE or KRAKEN2 feature-classifier , taxonomy , its , fungi	1	29	June 11, 2025
Feature table and representative sequences for downstream analyses feature-table	2	16	July 13, 2025
PERMANOVA and DESeq2: Re-evaluating Covariates After Subsetting by Time Point permanova	1	28	June 9, 2025
maarjAM database	3	41	June 6, 2025
Difficulty assigning taxonomy to cyanobacterial ASVs taxonomy , greengenes , silva , ncbi	2	47	June 5, 2025
Analysing with multiple public datasets	4	35	June 5, 2025
Qiime2 ConQuR Plugin for Batch Correction (or alternative) batch-effects	2	37	June 4, 2025
Shotgun sequencing and Trimming dada2	2	23	June 3, 2025
Can I run QIIME2 on de-multiplexed 5R 16S files?	1	20	June 3, 2025
Try to compare 16s and wgs with greengenes2	0	27	May 16, 2025

Frequent Questions and "Best of the QIIME 2 Forum"

best-of-the-forum

The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…