Latest General Discussion topics

Topic	Replies	Views	Activity
Frequent Questions and "Best of the QIIME 2 Forum" best-of-the-forum The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…	2	8210	February 27, 2020
About the General Discussion category	1	1005	March 13, 2023
How to use qiime for single and paired from different sequencing platforms with different read length?	1	22	April 13, 2026
Low overlap due to poor reverse read quality in 2×300 bp V3–V4 amplicon sequencing (DADA2) dada2	1	21	April 13, 2026
How to use qiime tools make-report (qiime2-amplicon-2025.10)	9	151	April 5, 2026
Comparing two data sets, where the first data set was sequence for V3-V4 and the second was only sequenced V4	1	39	March 26, 2026
q2-boots, q2-gemelli, rarefaction, and sampling depths gemelli , q2-boots	9	88	March 25, 2026
Can ANCOM-BC be used to analyse presence and absence data? ancom	3	120	March 21, 2026
Should alpha diversity with kmerizer be recalculated based on results of alpha-rarefaction? alpha-rarefaction , q2-kmerizer	6	63	March 21, 2026
processing ITS1& ITS2 samples	5	79	March 10, 2026
manifest file issue while doing 16s rna for small read in qiime2 import	3	36	March 9, 2026
Integrating V3-V4 and V4 16S datasets for large-scale meta-analysis: Is Greengenes2 the optimal choice for cross-region alignment? dada2 , feature-classifier , taxonomy , greengenes2	1	98	March 6, 2026
Many species have identical ITS fungal reference sequences in the ITS RefSeq database	3	50	March 4, 2026
Ancom-bc2 and use of structural zeros composition	1	36	March 3, 2026
BUSCO db fetching for MAG	6	46	February 26, 2026
Differential abundance with ANCOM-BC: is subsetting to a specific genus "legal" or just p-hacking? ancom-bc	4	79	February 19, 2026
Meta-analysis of multiple 16s datasets with different regions best practice?	6	111	February 10, 2026
Fungal primer choice avoiding plant amplification its	2	47	February 8, 2026
Hyperlink problem in gut-to-soil axis tutorial	7	61	February 6, 2026
Phylogenetic tree construction for ASV-based amplicon data	1	42	February 4, 2026
Best practice to identify mitochondria and chloroplasts using Greengenes2 2024.09 taxonomy	2	62	February 4, 2026
F-metric score in naive bayes classification of self-downloaded HROM microbiome database classifier	9	53	February 3, 2026
Alpha and Beta Diversity analysis using shotgun metagenomics data diversity , metagenomics	2	91	January 26, 2026
Comparing 16S and shotgun metagenomic data with greengenes2 metagenomics , greengenes2	4	122	January 20, 2026
bivalve gut microbiota, water and soil samples What OTU cluster? otus , closed-reference	11	89	January 19, 2026
Advice in best practice for handling multiple sequencing runs and subset analyses in QIIME 2 (DADA2) dada2 , feature-table	2	49	January 16, 2026
Quickstart not working in Safari, but works on Chrome	5	39	January 14, 2026
Seeking advice for a Thesis project	5	124	January 12, 2026
How to deal with possible cross-contamination by a crucial bacteria filtering , contaminant , decontam	2	54	February 8, 2026
Diversity analysis interpretation	1	42	December 17, 2025

Frequent Questions and "Best of the QIIME 2 Forum"

best-of-the-forum

The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…