General Discussion


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Frequent Questions and "Best of the QIIME 2 Forum"
best-of-the-forum

The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…

2 3251 February 27, 2020
About the General Discussion category
0 480 November 14, 2018
Simpson's index in qiime2
4 691 May 19, 2022
Trimming long reads to a shorter region for a methods comparison
trim ,  dada2
0 26 April 18, 2022
Alpha diversity metrics to compare MiSeq and PacBio
queued
0 16 May 17, 2022
ITSX for Fungi dada2 analysis
queued
0 19 May 13, 2022
Features vs. bag of words
0 59 April 12, 2022
Is it best to work on abundance tables of ASVs or taxa?
16s ,  asv ,  taxonomy ,  feature-table
3 47 May 6, 2022
% coverage for Bacteria and Archaea using universal primers 341F and 806R
4 33 May 5, 2022
HOMD Database for Fragment Insertion?
fragment-insertion
2 41 May 4, 2022
DADA2:bacteria and fungi have different effect.
dada2
4 57 May 3, 2022
No bacteroidales in just two samples.
taxonomy
3 30 May 2, 2022
qpcr abundance vs ASV species richness
2 63 April 30, 2022
ITS - fungal analysis
dada2
1 68 April 29, 2022
microbiome analysis based on variable in Qiime2 to R intergration
2 41 April 28, 2022
weighted vs unweighted unifrac?
emperor ,  unifrac ,  beta
1 78 April 27, 2022
Is there a difference between using 'feature-table filter-samples' and using filtered input data from the beginning?
import ,  feature-table
6 60 April 26, 2022
Can I analyse two types of data which are sequenced by different ways together ?
1 46 April 27, 2022
Databases for known bacteria-disease relationships
0 44 March 17, 2022
How to analyze sequence data with 2 primers targeting 2 different gene regions multiplexed into one run?
cutadapt ,  primers ,  taxonomy ,  dada2 ,  queued
0 31 April 22, 2022
16s rRNA sequencing for V-3-V4 hyper variable region?
6 113 April 21, 2022
Microbiome positive or negative control (16S rRNA V3-V4)
queued
1 54 April 21, 2022
ANCOM cutoff value default
2 38 April 16, 2022
Dividing relative abundances in ASV table
relative-frequency ,  feature-table
6 73 April 13, 2022
deciding where to truncate
truncate ,  dada2
2 60 April 10, 2022
Feature classifier method?
feature-classifier
1 40 April 6, 2022
Unit of measurement for sequence length: unit base pairs versus unit nucleotides
3 50 April 6, 2022
top taxa vs top phyla/genera/species; some insights?
feature-table
5 64 May 6, 2022
Making the classifier small
2 46 April 3, 2022
General metabarcoding dataset
3 51 April 3, 2022