Latest General Discussion topics

Topic	Replies	Views	Activity
Frequent Questions and "Best of the QIIME 2 Forum" best-of-the-forum The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…	2	7874	February 27, 2020
About the General Discussion category	1	984	March 13, 2023
Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis? dada2 , queued	0	12	September 12, 2025
Percentage of reads discarded after running DADA2 on NextSeq data dada2	1	27	August 25, 2025
Batch effects - Normalizing sequencing runs with different sequence counts	3	27	August 20, 2025
MOSHPIT: removing contaminant reads from metagenome data moshpit	11	166	August 18, 2025
Interpreting usefulness of sequence data from quality scores	3	21	August 15, 2025
How to account for uneven sampling taxonomy	2	50	August 9, 2025
Shall I need to remove ASV with prevelance in one sample? dada2 , beta , alpha	4	36	August 8, 2025
Exclude significant results from betadisper from adonis2?	0	39	July 19, 2025
16S SRNA feature-classifier , taxonomy , feature-table	1	36	August 2, 2025
Cage effect ANOVA	11	229	July 31, 2025
GSR database in QIIME2?	2	24	July 28, 2025
Problems with Taxonomic Assignment	1	22	July 25, 2025
Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)? dada2	4	30	July 22, 2025
Sequence and taxonomy files for Silva v138.2	2	60	July 21, 2025
Beginner's Project	2	24	July 21, 2025
Recommended Step for Merging Metagenomics Datasets merging , merge	1	47	July 16, 2025
Quality control for shotgun metagenomics quality-control	5	556	July 17, 2025
Merging qiime2 sample metadata with exterior taxonomic classifications taxonomy , feature-table	1	21	July 13, 2025
PR2: Division vs. Phylum	1	30	July 11, 2025
Rarefaction vs Rarefying and when to use them in your analysis pipeline q2-boots	14	323	July 9, 2025
Is it necessary to separate ASVs classified at genus level into their respective sequences in ANCOMBC2 or other differential abundance analysis? 16s , ancom-bc	2	48	July 8, 2025
Transforming Alpha Diversity Metrics for Mixed Effects Modeling	2	31	July 8, 2025
Best workflow for samples with high off-target (host) amplification	7	60	July 8, 2025
Greengenes2 vs. SILVA silva , greengenes2	0	68	July 3, 2025
High percentage of unassigned reads (metagenomics)	6	45	July 3, 2025
SILVA trained version for V3-V4 amplicon using 341F/785R primers	3	56	July 2, 2025
Looking for Sensible Qiime2 CLI Aliases q2cli	1	32	July 1, 2025
How to simplify the formula gini index in QIIME2? For example write in a R script. diversity , alpha	2	48	November 1, 2024

Frequent Questions and "Best of the QIIME 2 Forum"

best-of-the-forum

The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…