Latest General Discussion topics

Topic	Replies	Views	Activity
Frequent Questions and "Best of the QIIME 2 Forum" best-of-the-forum The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…	1	8348	February 20, 2020
About the General Discussion category	0	1016	November 14, 2018
kmers for taxonomy-free beta-diversity measures: an opportunity to integrate amplicon and shotgun analysis? q2-kmerizer	2	32	September 2, 2026
ASV vs OTU for fungal ITS its	11	1515	August 30, 2026
Is it bad to use multiple Qiime2 versions in one workflow?	2	35	August 29, 2026
Why truncate with dada2 when ITSxpress is an option? dada2 , q2-itsxpress	16	248	August 17, 2026
NCBI or SILVA for curating a custom 16S rDNA reference database?	1	58	August 12, 2026
option to rotate the barplots 90 degrees	6	97	August 3, 2026
Seeking Advice on Primer and Adapter Trimming for MiSeq Amplicon Sequencing Data Using Cutadapt cutadapt , trim	1	212	August 3, 2026
Sequencing Depth Control: Rarefying vs Rarifaction vs CLR vs Conditional Filtering alpha-rarefaction	0	82	July 31, 2026
Is my truncation parameter exploration sufficient, and is my interpretation correct? dada2	1	54	July 28, 2026
Identifying Contaminants Before Filtering: E Coli? filter-features	3	79	July 16, 2026
Sequencing platform fully confounded with study period: MiSeq vs NextSeq 2000 (16S rRNA V3-V4) dada2 , merging , batch-effects	3	86	July 7, 2026
Edible wild plants in human diet: effects on the gut microbiome	0	27	July 2, 2026
q2-boots, q2-gemelli, rarefaction, and sampling depths gemelli , q2-boots	8	180	June 22, 2026
Legend too long for Genus-level bar plots in QIIME2 (does not fit in Word/manuscript)	1	63	June 17, 2026
High number of OTUs/features in low-depth Nanopore 16S gastric biopsy data	4	88	June 11, 2026
Core metrics sugesttions	2	57	June 11, 2026
da-barplot-ancombc into inkscape	1	93	January 22, 2025
Running DADA2 on pre-processed data dada2	7	145	June 3, 2026
Processing Thermo Ion 16S Metagenomics Kit data in QIIME2: primer trimming, denoise-pyro, and SILVA classifier choice	4	73	May 28, 2026
Query about false read merging or no read merging dada2 , merge	1	41	May 26, 2026
Abundance overlap microbiome	2	63	May 25, 2026
Rarefaction vs Rarefying and when to use them in your analysis pipeline q2-boots	14	1962	May 22, 2026
Merging NGS with TGS? dada2 , taxonomy	3	65	May 20, 2026
Data analysis from Oxford Nanopore taxonomy	1	69	May 19, 2026
BLAST, VSEARCH or NB Classifier for 18S data with PR2 database?	1	105	May 13, 2026
sequence alignment dada2	5	78	May 11, 2026
quality control-script	1	42	May 8, 2026
Fragment-insertion SEPP for meta-analysis fragment-insertion	5	110	May 8, 2026

Frequent Questions and "Best of the QIIME 2 Forum"

best-of-the-forum

The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…