Latest General Discussion topics

Topic	Replies	Views	Activity
Frequent Questions and "Best of the QIIME 2 Forum" best-of-the-forum The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…	2	7742	February 27, 2020
About the General Discussion category	1	981	March 13, 2023
Greengenes2 vs. SILVA queued , silva , greengenes2	0	9	July 3, 2025
High percentage of unassigned reads (metagenomics)	6	14	July 3, 2025
SILVA trained version for V3-V4 amplicon using 341F/785R primers	3	14	July 2, 2025
Looking for Sensible Qiime2 CLI Aliases q2cli	1	31	July 1, 2025
Best workflow for samples with high off-target (host) amplification	4	23	June 28, 2025
How to simplify the formula gini index in QIIME2? For example write in a R script. diversity , alpha	2	45	November 1, 2024
Looking for Comprehensive Qiime2 Documentation	7	120	June 23, 2025
Request for Pre-trained SILVA V1–V3 Naive Bayes Classifier feature-classifier	2	32	June 18, 2025
classify-sklearn vs classify-consensus-vsearch - classification depth taxonomy	2	43	June 13, 2025
Shotgun sequencing of Fungi - UNITE or KRAKEN2 feature-classifier , taxonomy , its , fungi	1	28	June 11, 2025
Feature table and representative sequences for downstream analyses feature-table	1	15	June 11, 2025
Rarefaction vs Rarefying and when to use them in your analysis pipeline q2-boots	12	159	June 10, 2025
PERMANOVA and DESeq2: Re-evaluating Covariates After Subsetting by Time Point permanova	1	27	June 9, 2025
maarjAM database	3	38	June 6, 2025
Difficulty assigning taxonomy to cyanobacterial ASVs taxonomy , greengenes , silva , ncbi	2	46	June 5, 2025
Analysing with multiple public datasets	4	32	June 5, 2025
Qiime2 ConQuR Plugin for Batch Correction (or alternative) batch-effects	2	35	June 4, 2025
Shotgun sequencing and Trimming dada2	2	22	June 3, 2025
Can I run QIIME2 on de-multiplexed 5R 16S files?	1	19	June 3, 2025
Try to compare 16s and wgs with greengenes2	0	26	May 16, 2025
MAG filtering prior functional annotation moshpit , q2-annotate	2	55	May 28, 2025
Using PCoA versus PCA	8	11783	May 23, 2025
Can trimming and dada 2 dada2	3	38	May 19, 2025
Knowing how many sequences did not get given taxonomy	1	15	May 19, 2025
Would you like me to help with troubleshooting the differences between R analysis and the QIIME2 results?	1	31	May 19, 2025
how to select samples using stacked barplot taxonomy , barplot , filter-samples	2	27	May 7, 2025
Request for Bioinformatics Support: Alpha and Beta Diversity Analyses for 16S rRNA Nanopore Sequencing Data	0	58	May 5, 2025
Optimising spike-in quantity using qPCR primers	1	40	May 1, 2025

Frequent Questions and "Best of the QIIME 2 Forum"

best-of-the-forum

The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…