Latest General Discussion topics

Topic	Replies	Views	Activity
Frequent Questions and "Best of the QIIME 2 Forum" best-of-the-forum The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…	1	8309	February 20, 2020
About the General Discussion category	0	1011	November 14, 2018
Identifying Contaminants Before Filtering: E Coli? filter-features	3	52	July 16, 2026
Sequencing platform fully confounded with study period: MiSeq vs NextSeq 2000 (16S rRNA V3-V4) dada2 , merging , batch-effects	3	60	July 7, 2026
Edible wild plants in human diet: effects on the gut microbiome	0	23	July 2, 2026
q2-boots, q2-gemelli, rarefaction, and sampling depths gemelli , q2-boots	8	150	June 22, 2026
Legend too long for Genus-level bar plots in QIIME2 (does not fit in Word/manuscript)	1	51	June 17, 2026
High number of OTUs/features in low-depth Nanopore 16S gastric biopsy data	4	71	June 11, 2026
Core metrics sugesttions	2	44	June 11, 2026
da-barplot-ancombc into inkscape	1	90	January 22, 2025
Running DADA2 on pre-processed data dada2	7	99	June 3, 2026
ASV vs OTU for fungal ITS its	9	1434	May 30, 2026
Processing Thermo Ion 16S Metagenomics Kit data in QIIME2: primer trimming, denoise-pyro, and SILVA classifier choice	4	65	May 28, 2026
Query about false read merging or no read merging dada2 , merge	1	37	May 26, 2026
Abundance overlap microbiome	2	59	May 25, 2026
Rarefaction vs Rarefying and when to use them in your analysis pipeline q2-boots	14	1735	May 22, 2026
Merging NGS with TGS? dada2 , taxonomy	3	60	May 20, 2026
Data analysis from Oxford Nanopore taxonomy	1	54	May 19, 2026
BLAST, VSEARCH or NB Classifier for 18S data with PR2 database?	1	85	May 13, 2026
sequence alignment dada2	5	69	May 11, 2026
quality control-script	1	38	May 8, 2026
Fragment-insertion SEPP for meta-analysis fragment-insertion	5	89	May 8, 2026
Can I create one OTU table from mixed ITS1, ITS2, and full ITS fungal datasets? fungi , q2-itsxpress	5	62	May 6, 2026
ANCOM- BC pre-processing	8	103	May 6, 2026
Help - DADA2 microbiome analysis dada2 , 16s , qiime2	3	57	May 4, 2026
RESCRIPt custom database Silva vs NCBI taxonomy	6	76	April 23, 2026
How to use qiime tools make-report (qiime2-amplicon-2025.10)	5	226	April 21, 2026
How to use qiime for single and paired from different sequencing platforms with different read length?	1	36	April 13, 2026
Low overlap due to poor reverse read quality in 2×300 bp V3–V4 amplicon sequencing (DADA2) dada2	1	65	April 13, 2026
Comparing two data sets, where the first data set was sequence for V3-V4 and the second was only sequenced V4	1	52	March 26, 2026

Frequent Questions and "Best of the QIIME 2 Forum"

best-of-the-forum

The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…