Latest General Discussion topics

Topic	Replies	Views	Activity
Frequent Questions and "Best of the QIIME 2 Forum" best-of-the-forum The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…	2	8006	February 27, 2020
About the General Discussion category	1	991	March 13, 2023
Seeking alternatives to rarefaction for Alpha diversity analysis	3	37	December 3, 2025
Guidance on Merging and Handling Samples from Multiple Sequencing Runs	5	231	December 2, 2025
Lost files - rep-seq already filtered, need combined	1	7	November 25, 2025
priors in qiime2-dada2 dada2	4	244	November 18, 2025
How to find the genus level from bacteria with from 16s method	4	45	November 18, 2025
Phylogenetic tree: Reconstruct vs. Prune - When to? tree	6	81	November 15, 2025
DADA2 -- denoise dada2	2	53	November 7, 2025
MOSHPIT: removing contaminant reads from metagenome data moshpit	12	286	November 6, 2025
code “E.AniM” in KO	2	39	November 3, 2025
Filtering all Metazoa and bacteria out of my sample	1	39	October 23, 2025
PERMANOVA results significant, but differential abundance results detect 0 significantly different ASVs	3	71	October 20, 2025
Interpreting KEGG vs MetaCyc pathways in 16S (PICRUSt2) functional prediction dada2 , picrust	1	42	October 18, 2025
Rarefaction vs Rarefying and when to use them in your analysis pipeline q2-boots	16	682	October 16, 2025
Converting 16S V3–V4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?) dada2 , feature-table	3	76	October 9, 2025
How to test for dissimilarity for my samples	2	261	October 9, 2025
Rareifying ASV reads for Alpha and Beta diversity analysis	1	39	September 26, 2025
Ancom-BC2 analysis along time series ancom-bc2	4	74	September 24, 2025
Training a classifer for 18S and CO1 edna	2	41	September 21, 2025
Axis value percentages have no meaning in PCoA ?	5	62	September 20, 2025
Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis? dada2	4	101	September 18, 2025
Percentage of reads discarded after running DADA2 on NextSeq data dada2	1	49	August 25, 2025
Batch effects - Normalizing sequencing runs with different sequence counts	3	46	August 20, 2025
Interpreting usefulness of sequence data from quality scores	3	35	August 15, 2025
How to account for uneven sampling taxonomy	2	57	August 9, 2025
Shall I need to remove ASV with prevelance in one sample? dada2 , beta , alpha	4	63	August 8, 2025
Exclude significant results from betadisper from adonis2?	0	68	July 19, 2025
16S SRNA feature-classifier , taxonomy , feature-table	1	72	August 2, 2025
Cage effect ANOVA	11	263	July 31, 2025

Frequent Questions and "Best of the QIIME 2 Forum"

best-of-the-forum

The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…