Latest General Discussion topics

Topic	Replies	Views	Activity
Frequent Questions and "Best of the QIIME 2 Forum" best-of-the-forum The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…	2	8282	February 27, 2020
About the General Discussion category	1	1008	March 13, 2023
q2-boots, q2-gemelli, rarefaction, and sampling depths gemelli , q2-boots	10	131	June 22, 2026
Legend too long for Genus-level bar plots in QIIME2 (does not fit in Word/manuscript)	1	44	June 17, 2026
High number of OTUs/features in low-depth Nanopore 16S gastric biopsy data	4	65	June 11, 2026
Core metrics sugesttions	2	39	June 11, 2026
da-barplot-ancombc into inkscape	3	88	June 8, 2026
Running DADA2 on pre-processed data dada2	7	83	June 3, 2026
ASV vs OTU for fungal ITS its	9	1409	May 30, 2026
Processing Thermo Ion 16S Metagenomics Kit data in QIIME2: primer trimming, denoise-pyro, and SILVA classifier choice	4	55	May 28, 2026
Query about false read merging or no read merging dada2 , merge	1	35	May 26, 2026
Abundance overlap microbiome	2	57	May 25, 2026
Rarefaction vs Rarefying and when to use them in your analysis pipeline q2-boots	18	1635	May 22, 2026
Merging NGS with TGS? dada2 , taxonomy	3	56	May 20, 2026
Data analysis from Oxford Nanopore taxonomy	1	50	May 19, 2026
BLAST, VSEARCH or NB Classifier for 18S data with PR2 database?	1	81	May 13, 2026
sequence alignment dada2	5	66	May 11, 2026
quality control-script	1	36	May 8, 2026
Fragment-insertion SEPP for meta-analysis fragment-insertion	5	83	May 8, 2026
Can I create one OTU table from mixed ITS1, ITS2, and full ITS fungal datasets? fungi , q2-itsxpress	5	61	May 6, 2026
ANCOM- BC pre-processing	8	99	May 6, 2026
Help - DADA2 microbiome analysis dada2 , 16s , qiime2	3	53	May 4, 2026
RESCRIPt custom database Silva vs NCBI taxonomy	6	73	April 23, 2026
How to use qiime tools make-report (qiime2-amplicon-2025.10)	10	214	April 21, 2026
How to use qiime for single and paired from different sequencing platforms with different read length?	1	34	April 13, 2026
Low overlap due to poor reverse read quality in 2×300 bp V3–V4 amplicon sequencing (DADA2) dada2	1	61	April 13, 2026
Comparing two data sets, where the first data set was sequence for V3-V4 and the second was only sequenced V4	1	50	March 26, 2026
Can ANCOM-BC be used to analyse presence and absence data? ancom	3	134	March 21, 2026
Should alpha diversity with kmerizer be recalculated based on results of alpha-rarefaction? alpha-rarefaction , q2-kmerizer	6	91	March 21, 2026
processing ITS1& ITS2 samples	5	92	March 10, 2026

Frequent Questions and "Best of the QIIME 2 Forum"

best-of-the-forum

The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…