Latest General Discussion topics

Topic	Replies	Views	Activity
Frequent Questions and "Best of the QIIME 2 Forum" best-of-the-forum The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…	2	7550	February 27, 2020
About the General Discussion category	1	975	March 13, 2023
QIIME2 microbiome analysis and python version demux , fastq	1	12	March 27, 2025
Why do we get higher species level assignment with an older GTDB classifier than with a more recent one? dada2 , taxonomy , gtdb , greengenes2	6	48	March 27, 2025
TEF1 metabarcoding: Should I use BLAST or VSEARCH instead of Naive Bayes? taxonomy , tef1 , naivebayes	4	22	March 26, 2025
Investigating the Impact of Travel-Induced Environmental Shifts on Microbiome Stability: A Call for Collaborative Field Studies and Methodological Insights queued	0	14	March 26, 2025
DADA2 generated rep.seqs in R to QIIME2 for making use of the "qiime fragment-insertion sepp"	1	18	March 25, 2025
QIIME in clinical hospital and commercial laboratory settings	6	40	March 24, 2025
General or taxonomic level alpha diversity?	2	28	March 24, 2025
Reading the generated data.	1	18	March 24, 2025
Closed reference OTU picking of V3-V4 amplicon against full-length reference database closed-reference	3	17	March 21, 2025
Question about analyzing different sample types	3	40	March 20, 2025
SILVA vs PR2 database using qiime2 feature-classifier , taxonomy , silva , 18s	1	15	March 19, 2025
Contents of imported database taxonomy , rescript	6	20	March 18, 2025
Missing species sequence on Greengenes2 2024.09 greengenes2	2	15	March 18, 2025
How important is it to remove spacers prior to DADA2 filtering trimming, etc?	2	22	March 17, 2025
Comparison of OTU Identifications with Silva 138.1 and Greengenes 2022.10 databases feature-classifier , greengenes , silva	7	50	March 16, 2025
ASV with missing taxonomic annotation feature-classifier , taxonomy	2	29	March 11, 2025
Dual-End Barcoding for 16S rRNA Primers: Using Golay Barcodes on Both Forward and Reverse Primers barcode	4	33	March 10, 2025
How to download seven-number-summaries TSV directly dada2	1	12	March 7, 2025
PCoA analysis Interpretation Doubt	3	24	March 6, 2025
Is it valid to rename ASVs classified as ‘uncultivated’ as ‘unclassified’? taxonomy	9	37	March 5, 2025
OTU Tables Relative Frequency to Relative Abundance feature-table , relative-frequency	9	47	March 4, 2025
how to phylogenetically analyse 16S genes recovered from metagenome? taxonomy , phylogeny	2	40	March 3, 2025
Testing ITS1 Pipeline: Finding mock communities or correcting ITSxpress error q2-itsxpress	7	67	March 3, 2025
choosing of sampling depth dada2	7	72	February 28, 2025
Animals gene family dada2	1	15	February 26, 2025
tutorial for merging different sequencing data in qiime2 dada2	6	49	February 26, 2025
Sequencing bacterial 16S amplicons and Fungal ITS amplicons from same soil samples using same indices	2	27	February 26, 2025
Identification of Acanthamoeba in 18S rDNA analysis feature-classifier	1	12	February 24, 2025

Frequent Questions and "Best of the QIIME 2 Forum"

best-of-the-forum

The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…