Latest General Discussion topics

Topic	Replies	Views	Activity
Frequent Questions and "Best of the QIIME 2 Forum" best-of-the-forum The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…	1	8343	February 20, 2020
About the General Discussion category	0	1015	November 14, 2018
ASV vs OTU for fungal ITS its	11	1498	August 30, 2026
Is it bad to use multiple Qiime2 versions in one workflow?	2	12	August 29, 2026
Why truncate with dada2 when ITSxpress is an option? dada2 , q2-itsxpress	16	244	August 17, 2026
NCBI or SILVA for curating a custom 16S rDNA reference database?	1	54	August 12, 2026
option to rotate the barplots 90 degrees	6	96	August 3, 2026
Seeking Advice on Primer and Adapter Trimming for MiSeq Amplicon Sequencing Data Using Cutadapt cutadapt , trim	1	209	August 3, 2026
Sequencing Depth Control: Rarefying vs Rarifaction vs CLR vs Conditional Filtering alpha-rarefaction	0	73	July 31, 2026
Is my truncation parameter exploration sufficient, and is my interpretation correct? dada2	1	53	July 28, 2026
Identifying Contaminants Before Filtering: E Coli? filter-features	3	76	July 16, 2026
Sequencing platform fully confounded with study period: MiSeq vs NextSeq 2000 (16S rRNA V3-V4) dada2 , merging , batch-effects	3	81	July 7, 2026
Edible wild plants in human diet: effects on the gut microbiome	0	27	July 2, 2026
q2-boots, q2-gemelli, rarefaction, and sampling depths gemelli , q2-boots	8	174	June 22, 2026
Legend too long for Genus-level bar plots in QIIME2 (does not fit in Word/manuscript)	1	62	June 17, 2026
High number of OTUs/features in low-depth Nanopore 16S gastric biopsy data	4	85	June 11, 2026
Core metrics sugesttions	2	56	June 11, 2026
da-barplot-ancombc into inkscape	1	93	January 22, 2025
Running DADA2 on pre-processed data dada2	7	137	June 3, 2026
Processing Thermo Ion 16S Metagenomics Kit data in QIIME2: primer trimming, denoise-pyro, and SILVA classifier choice	4	72	May 28, 2026
Query about false read merging or no read merging dada2 , merge	1	41	May 26, 2026
Abundance overlap microbiome	2	63	May 25, 2026
Rarefaction vs Rarefying and when to use them in your analysis pipeline q2-boots	14	1937	May 22, 2026
Merging NGS with TGS? dada2 , taxonomy	3	65	May 20, 2026
Data analysis from Oxford Nanopore taxonomy	1	66	May 19, 2026
BLAST, VSEARCH or NB Classifier for 18S data with PR2 database?	1	104	May 13, 2026
sequence alignment dada2	5	78	May 11, 2026
quality control-script	1	41	May 8, 2026
Fragment-insertion SEPP for meta-analysis fragment-insertion	5	108	May 8, 2026
Can I create one OTU table from mixed ITS1, ITS2, and full ITS fungal datasets? fungi , q2-itsxpress	5	67	May 6, 2026

Frequent Questions and "Best of the QIIME 2 Forum"

best-of-the-forum

The following is a list of frequently asked questions on the forum, with answers, to serve as a point of reference for forum users to quickly find answers, or for forum users and moderators to use as a reference when hel…