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Advice on Conducting a Targeted Pathogenic Species Search using ITS1 data using QIIME2 feature-classifier plugin
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3
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68
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September 24, 2026
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16S Sequencing for Species-Level Resolution
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5
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212
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September 8, 2026
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pre-trained UNITE ITS classifiers for QIIME 2026.7 and scikit-learn=1.7.1
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15
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9463
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August 31, 2026
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Using RESCRIPt's 'extract-seq-segments' to extract reference sequences without PCR primer pairs.
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5
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6811
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November 14, 2023
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Train a taxonomy classifier by extracting an amplicon region from a curated sequence alignment (using SILVA as an example).
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0
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123
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August 12, 2026
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RESCRIPt-evaluate
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0
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105
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July 27, 2026
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Naive Bayes classifier trained on a custom NCBI RefSeq V3-V4 database classifies almost all ASVs as same species
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1
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83
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July 15, 2026
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ITS Classifier: Extract reference reads - HiSeq:
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2
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89
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May 31, 2026
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ITS2 classification: identical sequences but different results (DADA2 vs merged)
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6
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150
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April 7, 2026
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MIMt database for 16S, 18S, ITS, 23S and 28S new release available (March 26)
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0
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139
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March 18, 2026
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Annotation and Classifier question
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17
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262
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March 18, 2026
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process killed qiime 2024.5 on Ubuntu 24.04.4 LTS when running on multicore
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1
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76
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March 11, 2026
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Integrating V3-V4 and V4 16S datasets for large-scale meta-analysis: Is Greengenes2 the optimal choice for cross-region alignment?
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1
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189
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March 6, 2026
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QIIME2-amplicon-2025.7 16S PacBio classification issues/discussion
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6
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190
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February 24, 2026
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The differences in taxonomy output by the feature-classifier trainer and the full-length trainer
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6
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156
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January 18, 2026
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"over-classification" of ASV taxonomy?
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2
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106
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January 9, 2026
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How to perform SILVA V3 classifier training?
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9
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315
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November 17, 2025
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Troubleshoot classification where all ASVs are unassigned?
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7
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166
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December 2, 2025
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Evaluate custom taxonomic classifier with RESCRIPt?
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5
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210
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November 27, 2025
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MIMt database new release!!! QIIME2 classifiers for markers 16S, ITS, 18S, 23S and 28S
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0
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143
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November 3, 2025
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Feature-clasifier V3-V4
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6
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195
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October 21, 2025
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Trained classifier output not found
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6
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136
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September 11, 2025
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Question about consensus taxonomy assignment in classify-consensus-vsearch
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6
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235
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August 21, 2025
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Is there a pre-trained QIIME 2 classifier with full-length 16S (.qza)?
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10
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344
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August 14, 2025
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16S SRNA
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1
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108
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August 2, 2025
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Greengenes2 and RESCRIPt
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2
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167
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July 9, 2025
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SILVA 138.1 classifier trained on version 2023.5 and used in 2024.5
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2
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274
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July 3, 2025
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Dealing with Poor Taxonomic Assignments
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6
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226
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June 24, 2025
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Processing, filtering, and evaluating the SILVA database (and other reference sequence data) with RESCRIPt
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8
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66785
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February 17, 2023
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Request for Pre-trained SILVA V1–V3 Naive Bayes Classifier
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2
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113
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June 18, 2025
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