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Sequencing platform fully confounded with study period: MiSeq vs NextSeq 2000 (16S rRNA V3-V4)
|
|
0
|
15
|
July 1, 2026
|
|
QIIME 2 processing of MiSeq i100 data with constant quality scores
|
|
3
|
79
|
June 8, 2026
|
|
Running DADA2 on pre-processed data
|
|
7
|
86
|
June 3, 2026
|
|
Query about false read merging or no read merging
|
|
1
|
35
|
May 26, 2026
|
|
Merging NGS with TGS?
|
|
3
|
56
|
May 20, 2026
|
|
Under the hood of the "Moving Pictures" tutorial
|
|
0
|
123
|
May 13, 2026
|
|
sequence alignment
|
|
5
|
66
|
May 11, 2026
|
|
An error was encountered while running DADA2 in R (return code 1), inspect stdout and stderr to learn more
|
|
13
|
114
|
June 8, 2026
|
|
Help - DADA2 microbiome analysis
|
|
3
|
53
|
May 4, 2026
|
|
Qiime version and singletons
|
|
4
|
66
|
May 29, 2026
|
|
High number of unique sequences in PacBio full-length 16S CCS data with DADA2
|
|
2
|
99
|
April 24, 2026
|
|
Low overlap due to poor reverse read quality in 2×300 bp V3–V4 amplicon sequencing (DADA2)
|
|
1
|
61
|
April 13, 2026
|
|
High but variable sequence loss (32-68%) during DADA2 filtering with V3-V4 amplicons (MiSeq 600 cycles)
|
|
3
|
102
|
May 10, 2026
|
|
Inadequate overlap for V3-V4 (paired-end) due to quality drop
|
|
6
|
105
|
April 10, 2026
|
|
Integrating V3-V4 and V4 16S datasets for large-scale meta-analysis: Is Greengenes2 the optimal choice for cross-region alignment?
|
|
1
|
133
|
March 6, 2026
|
|
Too many elements for DADA2 - denoise in batches?
|
|
7
|
137
|
April 5, 2026
|
|
Analysis with DADA2... Merge Read Length
|
|
2
|
49
|
April 4, 2026
|
|
denoise failure
|
|
6
|
72
|
March 28, 2026
|
|
Huge difference of DADA2 output with and without removing primers
|
|
5
|
104
|
February 27, 2026
|
|
Advice in best practice for handling multiple sequencing runs and subset analyses in QIIME 2 (DADA2)
|
|
2
|
89
|
January 16, 2026
|
|
pair ended demultiplexed qza file causing strange error.
|
|
2
|
59
|
January 29, 2026
|
|
High read loss due to artifacts and chimera after denoising 16S V3-V4 paired-end reads
|
|
2
|
66
|
January 17, 2026
|
|
Denoising low quality reverse reads in paired-end sequencing reads
|
|
5
|
86
|
January 3, 2026
|
|
Issue with UNITE classifier for ITS1
|
|
2
|
79
|
January 2, 2026
|
|
Error was encountered while running DADA2 in R (return code 1)
|
|
9
|
101
|
January 5, 2026
|
|
DADA2 denoising taking long time
|
|
7
|
127
|
January 1, 2026
|
|
Questions about performing quality control with dada2 and generating a feature table
|
|
3
|
51
|
December 30, 2025
|
|
DADA2 priors trimming
|
|
1
|
35
|
November 18, 2025
|
|
priors in qiime2-dada2
|
|
4
|
265
|
November 18, 2025
|
|
QIIME2 non-chimeric reads
|
|
4
|
85
|
December 11, 2025
|
