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Why truncate with dada2 when ITSxpress is an option?
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12
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144
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August 4, 2026
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Request: DADA2 denoise-ccs without --p-front parameter
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2
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39
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August 4, 2026
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PacBio ITS workflow - some questions
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2
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62
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July 28, 2026
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Is my truncation parameter exploration sufficient, and is my interpretation correct?
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1
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48
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July 28, 2026
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Losing reads at DADA2 Merge step 30% of my samples lost over 50% - I have tried multiple steps to fix- please help
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1
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34
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July 21, 2026
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Can DADA2 be used with nearly uniform Q38 quality scores?
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2
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49
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July 21, 2026
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Sequencing platform fully confounded with study period: MiSeq vs NextSeq 2000 (16S rRNA V3-V4)
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3
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77
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July 7, 2026
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QIIME 2 processing of MiSeq i100 data with constant quality scores
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3
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120
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June 8, 2026
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Running DADA2 on pre-processed data
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7
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127
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June 3, 2026
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Query about false read merging or no read merging
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1
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40
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May 26, 2026
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Merging NGS with TGS?
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3
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64
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May 20, 2026
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Under the hood of the "Moving Pictures" tutorial
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0
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154
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May 13, 2026
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sequence alignment
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5
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75
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May 11, 2026
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An error was encountered while running DADA2 in R (return code 1), inspect stdout and stderr to learn more
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10
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129
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May 8, 2026
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Help - DADA2 microbiome analysis
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3
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71
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May 4, 2026
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Qiime version and singletons
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3
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79
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April 27, 2026
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High number of unique sequences in PacBio full-length 16S CCS data with DADA2
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2
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120
|
April 24, 2026
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Low overlap due to poor reverse read quality in 2×300 bp V3–V4 amplicon sequencing (DADA2)
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1
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74
|
April 13, 2026
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High but variable sequence loss (32-68%) during DADA2 filtering with V3-V4 amplicons (MiSeq 600 cycles)
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2
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109
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April 9, 2026
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Inadequate overlap for V3-V4 (paired-end) due to quality drop
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5
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121
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March 9, 2026
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Integrating V3-V4 and V4 16S datasets for large-scale meta-analysis: Is Greengenes2 the optimal choice for cross-region alignment?
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1
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153
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March 6, 2026
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Too many elements for DADA2 - denoise in batches?
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6
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140
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March 4, 2026
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Analysis with DADA2... Merge Read Length
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1
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57
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March 4, 2026
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denoise failure
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5
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72
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February 24, 2026
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Huge difference of DADA2 output with and without removing primers
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4
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114
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January 27, 2026
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Advice in best practice for handling multiple sequencing runs and subset analyses in QIIME 2 (DADA2)
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2
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119
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January 16, 2026
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pair ended demultiplexed qza file causing strange error.
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1
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61
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December 28, 2025
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High read loss due to artifacts and chimera after denoising 16S V3-V4 paired-end reads
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1
|
74
|
December 17, 2025
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Denoising low quality reverse reads in paired-end sequencing reads
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4
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95
|
December 3, 2025
|
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Issue with UNITE classifier for ITS1
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1
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82
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December 2, 2025
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