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High but variable sequence loss (32-68%) during DADA2 filtering with V3-V4 amplicons (MiSeq 600 cycles)
|
|
2
|
24
|
April 9, 2026
|
|
Problem with cache using qiime cutadapt
|
|
10
|
54
|
March 30, 2026
|
|
Q2-cutadapt add "--nextseq-trim" option
|
|
3
|
71
|
February 27, 2026
|
|
Possible bug in qiime cutadapt demux paired (2024.10)
|
|
6
|
75
|
February 28, 2026
|
|
Huge difference of DADA2 output with and without removing primers
|
|
5
|
85
|
February 27, 2026
|
|
qiime cutadapt does not have an untrimmed sequences output
|
|
5
|
117
|
January 1, 2026
|
|
ITS denoising truncation
|
|
2
|
60
|
November 15, 2025
|
|
Duplicate barcode error, but barcodes listed are not the same.
|
|
7
|
90
|
September 20, 2025
|
|
cutadapt outside of QIIME2
|
|
1
|
55
|
August 4, 2025
|
|
Inconsistent results with discard untrimmed reads in cutadapt
|
|
7
|
118
|
August 10, 2025
|
|
QIIME 2 Meta-Analysis of Rhizosphere Microbiome with Mixed V3-V4/V4 Data: Best Practices for Downstream Analysis
|
|
14
|
472
|
May 18, 2025
|
|
Analysing V4/V5 16S rRNA illumina Miseq paired-end reads.
|
|
16
|
369
|
April 19, 2025
|
|
Trimming Paired-End Sequences with cutadapt While Maintaining Pairing Integrity
|
|
2
|
72
|
March 13, 2025
|
|
Which position is best for trimming in a paired-end approach?
|
|
11
|
143
|
February 25, 2025
|
|
Comparing amplicon studies that use different primers
|
|
9
|
180
|
December 22, 2024
|
|
Trimming 5bp in QIIME2 2024.5 Amplicon
|
|
4
|
41
|
December 16, 2024
|
|
Error messages after cutadapt, but got trimmed files.
|
|
5
|
130
|
December 8, 2024
|
|
Cut adapt results in empty files
|
|
4
|
113
|
November 19, 2024
|
|
One vs two adaptor sequences using cutadapt
|
|
4
|
99
|
September 27, 2024
|
|
QIIME2 Demultiplexing Sequence
|
|
7
|
202
|
October 4, 2024
|
|
V3-V4 16s - variable size after merging
|
|
7
|
236
|
September 6, 2024
|
|
problem with cutadapt trim-single
|
|
6
|
137
|
August 22, 2024
|
|
Seeking Advice on Primer and Adapter Trimming for MiSeq Amplicon Sequencing Data Using Cutadapt
|
|
0
|
168
|
June 4, 2024
|
|
Removal of Illumina Adapters
|
|
1
|
192
|
May 30, 2024
|
|
Error during deblur process
|
|
8
|
302
|
June 12, 2024
|
|
Cutadapt - trimming adaptor and primer sequences
|
|
3
|
224
|
June 11, 2024
|
|
Missing plugins to load Artifact to run cut adapt
|
|
2
|
141
|
June 8, 2024
|
|
Primer show up again after removal using cutadapt in qiime2 DADA2
|
|
11
|
404
|
May 6, 2024
|
|
I fell into the swamp of 273bp after trimming adapters and primers through cutadapt...
|
|
8
|
241
|
April 18, 2024
|
|
Pyrosequence work in Qiime2 (Barcode)
|
|
11
|
276
|
April 15, 2024
|
