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Seeking Advice on Primer and Adapter Trimming for MiSeq Amplicon Sequencing Data Using Cutadapt
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1
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224
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August 3, 2026
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How much do i have to trimming the dada2
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3
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236
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May 14, 2024
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I fell into the swamp of 273bp after trimming adapters and primers through cutadapt...
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7
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297
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March 18, 2024
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How to trim ransposase Adapters using qiime cutadapt or dada2 ?
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3
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426
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December 19, 2023
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ITS itsxpress trim-unmerged encounters problems
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1
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298
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September 13, 2023
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Deciding trim length for deblur
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2
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348
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July 31, 2023
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Demultiplexing and Trimming Adapters from Reads with q2-cutadapt
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4
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51053
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March 12, 2020
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How to remove primer and index from demux.qza
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13
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1265
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May 12, 2023
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Interpretation of quality plots
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9
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1152
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March 20, 2023
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Trimming long reads to a shorter region for a methods comparison
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0
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514
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April 18, 2022
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data trimming - a) Denoising and truncating
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2
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763
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April 14, 2022
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Nomenclature issue: trimming vs truncation
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5
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4758
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October 28, 2021
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Data loss when trimming ITS
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3
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562
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September 23, 2021
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Debular trim length
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3
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433
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September 16, 2021
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DADA2 error (same error for every try)
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0
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677
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July 29, 2021
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trimming read files containing a mixture of forward and reverse primers
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4
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1710
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June 7, 2021
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How to determine the values of trim parameters?
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1
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933
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February 2, 2021
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Lost primers / barcodes information
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4
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1226
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December 3, 2020
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Trimming and joining short reads resulted in few merged read on v4 region
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5
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2135
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August 4, 2020
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Trimming MiSeq sequencing data individually
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3
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937
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July 22, 2020
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Illumina demultiplexed data with inline index sequences (index5+index7)
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5
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2264
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January 14, 2020
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Trimming length in dada2 in PE data (2 x 300 bp)
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2
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1249
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December 4, 2019
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Would there be a reason to truncate the forward and reverse reads at different points?
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6
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2145
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September 5, 2019
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Low sequence count after DADA2
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5
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3167
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January 8, 2019
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How to clean Nextera paired-end libraries
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1
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1841
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November 27, 2018
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Cutadapt trim-paired odd result
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6
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1623
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November 16, 2018
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Where to trim reads
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24
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4695
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August 30, 2018
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16s V1-V3, V3-V4 and V4-V5 region with 2x300 MiSeq run
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5
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4434
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August 15, 2018
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Truncating reads with multiple sequencing runs and different primers
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1
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1799
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July 10, 2018
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DEBLUR -p-trim-length
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11
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5511
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February 27, 2018
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