Hi, my quality socre plots are:
But I lost the information of primers / barcodes for these paired sequencing.
So I don’t know how to do trim procedure.
I have some questions and need for helps:
- Is it any way to find the ‘unknown’ primers or barcodes?
- If I skip trim procedure, it is accurate if I trim the left sequencing at the denoise procedure? For example, use --p-trim-left-f = 32 and --p-trim-left-r = 9 to trim the primers.
Thanks in advance!