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question about primer trimming in qiime2 16S Miseq tutorials
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9
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107
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May 15, 2026
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Inconsistent results with discard untrimmed reads in cutadapt
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7
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124
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August 10, 2025
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Optimising spike-in quantity using qPCR
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1
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92
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May 1, 2025
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Primer orientations in illumina miseq metabarcoding data
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2
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231
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April 30, 2024
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How to trim ransposase Adapters using qiime cutadapt or dada2 ?
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6
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384
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March 1, 2024
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merging sequence runs with slight primer variation
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17
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503
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October 2, 2023
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Questions about 16S data from Novogene (UK)
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29
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2795
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September 20, 2023
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How do I check adapter and primer presence in sequences?
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7
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3911
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August 17, 2023
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How to remove primer and index from demux.qza
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16
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1204
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June 12, 2023
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Dada2 trimming for illumina 341F/805R primers
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4
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1380
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December 15, 2022
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N and H nucleotides in V3-V4 primers and cutadapt trimming
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7
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527
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December 4, 2022
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How to analyze sequence data with 2 primers targeting 2 different gene regions multiplexed into one run?
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2
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1185
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May 25, 2022
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Why we have to set trim-left parameter to 15 in analyzing Ion Torrent sequences data?
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3
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380
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May 7, 2022
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Truncate length for V3-V4 sequencing in dada2 process
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2
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988
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April 23, 2022
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Using different primer sets to amplify two different hypervariable regions on one illumina run
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6
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1043
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March 27, 2022
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dada2 paired / with vs without primer / merged huge loss
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6
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1502
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January 10, 2022
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Primer naming inconsistencies?
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2
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1053
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September 29, 2021
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Trying to extract reads and it's taking a VERY long time
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2
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641
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August 27, 2021
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Removing Primers: Cutadapt vs Denoising
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3
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4345
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August 3, 2021
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Questions about V3–V4 primers for 16S rRNA amplicon sequencing, and calculating overlap
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3
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22307
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August 21, 2021
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Feature classifier with multiple primers
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4
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859
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August 1, 2021
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trimming read files containing a mixture of forward and reverse primers
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4
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1680
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June 7, 2021
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Min and Max primer length
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2
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473
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June 25, 2021
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Merging closed OTU and denovo OTU
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7
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754
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May 30, 2021
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Primers & adapters missing
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3
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1134
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May 17, 2021
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Sequencing dataset with four different primers- Should I merge the datasets?
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1
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868
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March 6, 2021
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Do the EMP 18S rRNA primers amplify bacteria as well?
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4
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2286
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November 10, 2020
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On two methods of dealing with read-through sequences in q2-cutadapt
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4
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947
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November 24, 2020
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impact of quality primer on the quality of data
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3
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694
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November 10, 2019
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To remove primers and spacers or not? - Zero usable reads after DADA2
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16
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5104
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November 1, 2019
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