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Inconsistent results with discard untrimmed reads in cutadapt
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7
|
117
|
August 10, 2025
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Optimising spike-in quantity using qPCR
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1
|
88
|
May 1, 2025
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Primer orientations in illumina miseq metabarcoding data
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2
|
228
|
April 30, 2024
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How to trim ransposase Adapters using qiime cutadapt or dada2 ?
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6
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378
|
March 1, 2024
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merging sequence runs with slight primer variation
|
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17
|
490
|
October 2, 2023
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Questions about 16S data from Novogene (UK)
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29
|
2741
|
September 20, 2023
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How do I check adapter and primer presence in sequences?
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7
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3862
|
August 17, 2023
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How to remove primer and index from demux.qza
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16
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1188
|
June 12, 2023
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Dada2 trimming for illumina 341F/805R primers
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4
|
1359
|
December 15, 2022
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N and H nucleotides in V3-V4 primers and cutadapt trimming
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7
|
520
|
December 4, 2022
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How to analyze sequence data with 2 primers targeting 2 different gene regions multiplexed into one run?
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2
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1173
|
May 25, 2022
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Why we have to set trim-left parameter to 15 in analyzing Ion Torrent sequences data?
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|
3
|
378
|
May 7, 2022
|
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Truncate length for V3-V4 sequencing in dada2 process
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2
|
983
|
April 23, 2022
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|
Using different primer sets to amplify two different hypervariable regions on one illumina run
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6
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1041
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March 27, 2022
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dada2 paired / with vs without primer / merged huge loss
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6
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1497
|
January 10, 2022
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Primer naming inconsistencies?
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2
|
1044
|
September 29, 2021
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Trying to extract reads and it's taking a VERY long time
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2
|
637
|
August 27, 2021
|
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Removing Primers: Cutadapt vs Denoising
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3
|
4312
|
August 3, 2021
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Questions about V3–V4 primers for 16S rRNA amplicon sequencing, and calculating overlap
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3
|
22047
|
August 21, 2021
|
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Feature classifier with multiple primers
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4
|
856
|
August 1, 2021
|
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trimming read files containing a mixture of forward and reverse primers
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4
|
1673
|
June 7, 2021
|
|
Min and Max primer length
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|
2
|
464
|
June 25, 2021
|
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Merging closed OTU and denovo OTU
|
|
7
|
734
|
May 30, 2021
|
|
Primers & adapters missing
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|
3
|
1127
|
May 17, 2021
|
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Sequencing dataset with four different primers- Should I merge the datasets?
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1
|
862
|
March 6, 2021
|
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Do the EMP 18S rRNA primers amplify bacteria as well?
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4
|
2258
|
November 10, 2020
|
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On two methods of dealing with read-through sequences in q2-cutadapt
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|
4
|
939
|
November 24, 2020
|
|
impact of quality primer on the quality of data
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|
3
|
690
|
November 10, 2019
|
|
To remove primers and spacers or not? - Zero usable reads after DADA2
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|
16
|
5091
|
November 1, 2019
|
|
Clarification on primers, are a portion of them left in this run?
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|
5
|
2208
|
October 29, 2019
|