Hello All,
I tried running the script below to trim the first 5bp for my sequences and got the error in the screenshot below. I checked the cutadapt version 4.9 is installed.
qiime cutadapt trim-single \
--i-demultiplexed-sequences single-end-demux.qza \
--p-front 5 \
--o-trimmed-sequences single-end-demux-trimmed.qza
Plugin error from cutadapt:
Hi @jen.herting,
Welcome to the :qiime2: forum!
Can you re run your command with the --verbose parameter and then post your command and the error message here? A screenshot is great! That will give me more information on whats causing the problem here.
--Hannah
Okay @jen.herting,
Your issue is in the snippet above, it appears that there is the number 5 in your adaptor sequence, which is not an allowed character.
To fix this I would recommend following the guidelines in this forum post:
That should fix the issue. If you run into anything else or that doesn't work for you, we are here to help
--Hannah