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Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis?
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4
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125
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September 18, 2025
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Percentage of reads discarded after running DADA2 on NextSeq data
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1
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58
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August 25, 2025
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Batch effects - Normalizing sequencing runs with different sequence counts
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3
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64
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August 20, 2025
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Interpreting usefulness of sequence data from quality scores
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3
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42
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August 15, 2025
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How to account for uneven sampling
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2
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62
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August 9, 2025
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Shall I need to remove ASV with prevelance in one sample?
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4
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79
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August 8, 2025
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Exclude significant results from betadisper from adonis2?
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0
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80
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July 19, 2025
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16S SRNA
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1
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75
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August 2, 2025
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Cage effect ANOVA
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11
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278
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July 31, 2025
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GSR database in QIIME2?
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2
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43
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July 28, 2025
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Problems with Taxonomic Assignment
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1
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36
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July 25, 2025
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Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)?
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4
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118
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July 22, 2025
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Sequence and taxonomy files for Silva v138.2
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2
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122
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July 21, 2025
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Beginner's Project
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2
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52
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July 21, 2025
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Recommended Step for Merging Metagenomics Datasets
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1
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63
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July 16, 2025
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Quality control for shotgun metagenomics
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5
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624
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July 17, 2025
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Merging qiime2 sample metadata with exterior taxonomic classifications
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1
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48
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July 13, 2025
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PR2: Division vs. Phylum
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1
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67
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July 11, 2025
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Is it necessary to separate ASVs classified at genus level into their respective sequences in ANCOMBC2 or other differential abundance analysis?
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2
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103
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July 8, 2025
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Transforming Alpha Diversity Metrics for Mixed Effects Modeling
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2
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54
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July 8, 2025
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Best workflow for samples with high off-target (host) amplification
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7
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104
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July 8, 2025
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High percentage of unassigned reads (metagenomics)
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6
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111
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July 3, 2025
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SILVA trained version for V3-V4 amplicon using 341F/785R primers
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3
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182
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July 2, 2025
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Looking for Sensible Qiime2 CLI Aliases
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1
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44
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July 1, 2025
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How to simplify the formula gini index in QIIME2? For example write in a R script.
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2
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56
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November 1, 2024
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Looking for Comprehensive Qiime2 Documentation
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7
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162
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June 23, 2025
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Request for Pre-trained SILVA V1–V3 Naive Bayes Classifier
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3
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82
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July 20, 2025
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classify-sklearn vs classify-consensus-vsearch - classification depth
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2
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96
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June 13, 2025
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Shotgun sequencing of Fungi - UNITE or KRAKEN2
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1
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77
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June 11, 2025
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Feature table and representative sequences for downstream analyses
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2
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37
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July 13, 2025
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