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manifest file issue while doing 16s rna for small read in qiime2
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3
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48
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March 9, 2026
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Integrating V3-V4 and V4 16S datasets for large-scale meta-analysis: Is Greengenes2 the optimal choice for cross-region alignment?
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1
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129
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March 6, 2026
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Many species have identical ITS fungal reference sequences in the ITS RefSeq database
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3
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71
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March 4, 2026
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Ancom-bc2 and use of structural zeros
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1
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71
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March 3, 2026
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BUSCO db fetching for MAG
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6
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57
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February 26, 2026
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Differential abundance with ANCOM-BC: is subsetting to a specific genus "legal" or just p-hacking?
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4
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93
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February 19, 2026
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Meta-analysis of multiple 16s datasets with different regions best practice?
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6
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156
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February 10, 2026
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Fungal primer choice avoiding plant amplification
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2
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88
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February 8, 2026
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Hyperlink problem in gut-to-soil axis tutorial
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7
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75
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February 6, 2026
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Phylogenetic tree construction for ASV-based amplicon data
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1
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58
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February 4, 2026
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Best practice to identify mitochondria and chloroplasts using Greengenes2 2024.09
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2
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83
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February 4, 2026
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F-metric score in naive bayes classification of self-downloaded HROM microbiome database
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9
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59
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February 3, 2026
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Alpha and Beta Diversity analysis using shotgun metagenomics data
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2
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143
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January 26, 2026
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Comparing 16S and shotgun metagenomic data with greengenes2
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4
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177
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January 20, 2026
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bivalve gut microbiota, water and soil samples What OTU cluster?
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11
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111
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January 19, 2026
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Advice in best practice for handling multiple sequencing runs and subset analyses in QIIME 2 (DADA2)
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2
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85
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January 16, 2026
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Quickstart not working in Safari, but works on Chrome
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6
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47
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May 7, 2026
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Seeking advice for a Thesis project
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5
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128
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January 12, 2026
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How to deal with possible cross-contamination by a crucial bacteria
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2
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64
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February 8, 2026
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Diversity analysis interpretation
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1
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48
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December 17, 2025
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Remove Unassigned ASV for Tree construction
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3
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64
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December 16, 2025
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Seeking alternatives to rarefaction for Alpha diversity analysis
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8
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143
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December 11, 2025
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Guidance on Merging and Handling Samples from Multiple Sequencing Runs
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5
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475
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December 2, 2025
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Lost files - rep-seq already filtered, need combined
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1
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18
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November 25, 2025
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priors in qiime2-dada2
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4
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261
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November 18, 2025
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How to find the genus level from bacteria with from 16s method
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4
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85
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November 18, 2025
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Phylogenetic tree: Reconstruct vs. Prune - When to?
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6
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146
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November 15, 2025
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DADA2 -- denoise
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2
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71
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November 7, 2025
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MOSHPIT: removing contaminant reads from metagenome data
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12
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466
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November 6, 2025
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code “E.AniM” in KO
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2
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65
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November 3, 2025
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