DADA2 generated rep.seqs in R to QIIME2 for making use of the "qiime fragment-insertion sepp"
|
|
1
|
27
|
March 25, 2025
|
General or taxonomic level alpha diversity?
|
|
2
|
36
|
March 24, 2025
|
Reading the generated data.
|
|
1
|
23
|
March 24, 2025
|
Closed reference OTU picking of V3-V4 amplicon against full-length reference database
|
|
3
|
31
|
March 21, 2025
|
Question about analyzing different sample types
|
|
3
|
45
|
March 20, 2025
|
SILVA vs PR2 database using qiime2
|
|
1
|
62
|
March 19, 2025
|
Contents of imported database
|
|
6
|
25
|
March 18, 2025
|
Missing species sequence on Greengenes2 2024.09
|
|
2
|
31
|
March 18, 2025
|
How important is it to remove spacers prior to DADA2 filtering trimming, etc?
|
|
2
|
26
|
March 17, 2025
|
Comparison of OTU Identifications with Silva 138.1 and Greengenes 2022.10 databases
|
|
7
|
79
|
March 16, 2025
|
ASV with missing taxonomic annotation
|
|
2
|
56
|
March 11, 2025
|
Dual-End Barcoding for 16S rRNA Primers: Using Golay Barcodes on Both Forward and Reverse Primers
|
|
5
|
41
|
April 10, 2025
|
How to download seven-number-summaries TSV directly
|
|
1
|
17
|
March 7, 2025
|
PCoA analysis Interpretation Doubt
|
|
3
|
34
|
March 6, 2025
|
Is it valid to rename ASVs classified as ‘uncultivated’ as ‘unclassified’?
|
|
9
|
42
|
March 5, 2025
|
OTU Tables Relative Frequency to Relative Abundance
|
|
9
|
61
|
March 4, 2025
|
how to phylogenetically analyse 16S genes recovered from metagenome?
|
|
2
|
49
|
March 3, 2025
|
Testing ITS1 Pipeline: Finding mock communities or correcting ITSxpress error
|
|
7
|
76
|
March 3, 2025
|
choosing of sampling depth
|
|
7
|
77
|
February 28, 2025
|
Animals gene family
|
|
1
|
17
|
February 26, 2025
|
tutorial for merging different sequencing data in qiime2
|
|
6
|
56
|
February 26, 2025
|
Sequencing bacterial 16S amplicons and Fungal ITS amplicons from same soil samples using same indices
|
|
2
|
35
|
February 26, 2025
|
Identification of Acanthamoeba in 18S rDNA analysis
|
|
1
|
17
|
February 24, 2025
|
Recommended timepoints for soil microbiome studies?
|
|
5
|
43
|
February 19, 2025
|
Code Review: Custom QIIME 2
|
|
5
|
44
|
March 22, 2025
|
Can MiSeq Reagent Kit v2 (500 cycles) be used to sequence V3-V4 region of bacterial 16S rRNA gene?
|
|
4
|
53
|
February 19, 2025
|
How to deal with multiple runs for one sample?
|
|
8
|
41
|
February 18, 2025
|
Nanopore Long Read Demultiplexed Full 16S rRNA Seq
|
|
8
|
628
|
February 17, 2025
|
Issue with Taxonomy Assignment Using Kraken and Custom Database (BOLD, Nemabiome) in QIIME2
|
|
4
|
136
|
February 14, 2025
|
Uneven sample size, a need for rarefaction tool
|
|
9
|
100
|
February 11, 2025
|