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RESCRIPt custom database Silva vs NCBI taxonomy
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6
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89
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April 23, 2026
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How to use qiime tools make-report (qiime2-amplicon-2025.10)
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5
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236
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April 21, 2026
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How to use qiime for single and paired from different sequencing platforms with different read length?
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1
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40
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April 13, 2026
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Low overlap due to poor reverse read quality in 2×300 bp V3–V4 amplicon sequencing (DADA2)
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1
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74
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April 13, 2026
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Comparing two data sets, where the first data set was sequence for V3-V4 and the second was only sequenced V4
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1
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55
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March 26, 2026
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Can ANCOM-BC be used to analyse presence and absence data?
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3
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138
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March 21, 2026
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Should alpha diversity with kmerizer be recalculated based on results of alpha-rarefaction?
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6
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112
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March 21, 2026
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processing ITS1& ITS2 samples
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5
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102
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March 10, 2026
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manifest file issue while doing 16s rna for small read in qiime2
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3
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61
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March 9, 2026
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Integrating V3-V4 and V4 16S datasets for large-scale meta-analysis: Is Greengenes2 the optimal choice for cross-region alignment?
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1
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152
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March 6, 2026
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Many species have identical ITS fungal reference sequences in the ITS RefSeq database
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3
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118
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March 4, 2026
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Ancom-bc2 and use of structural zeros
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1
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98
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March 3, 2026
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BUSCO db fetching for MAG
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6
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71
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February 26, 2026
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Differential abundance with ANCOM-BC: is subsetting to a specific genus "legal" or just p-hacking?
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4
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109
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February 19, 2026
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Meta-analysis of multiple 16s datasets with different regions best practice?
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6
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195
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February 10, 2026
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Fungal primer choice avoiding plant amplification
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2
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115
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February 8, 2026
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Hyperlink problem in gut-to-soil axis tutorial
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7
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94
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February 6, 2026
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Phylogenetic tree construction for ASV-based amplicon data
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1
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68
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February 4, 2026
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Best practice to identify mitochondria and chloroplasts using Greengenes2 2024.09
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2
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110
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February 4, 2026
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F-metric score in naive bayes classification of self-downloaded HROM microbiome database
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9
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86
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February 3, 2026
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Alpha and Beta Diversity analysis using shotgun metagenomics data
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2
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193
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January 26, 2026
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Comparing 16S and shotgun metagenomic data with greengenes2
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3
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195
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January 20, 2026
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bivalve gut microbiota, water and soil samples What OTU cluster?
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11
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125
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January 19, 2026
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Advice in best practice for handling multiple sequencing runs and subset analyses in QIIME 2 (DADA2)
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2
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118
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January 16, 2026
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Quickstart not working in Safari, but works on Chrome
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4
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60
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January 14, 2026
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Seeking advice for a Thesis project
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3
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142
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January 12, 2026
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How to deal with possible cross-contamination by a crucial bacteria
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1
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68
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January 8, 2026
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Diversity analysis interpretation
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1
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55
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December 17, 2025
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Remove Unassigned ASV for Tree construction
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3
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73
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December 16, 2025
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Seeking alternatives to rarefaction for Alpha diversity analysis
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8
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162
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December 11, 2025
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