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Converting 16S V3–V4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?)
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3
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118
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October 9, 2025
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How to test for dissimilarity for my samples
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2
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273
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October 9, 2025
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Rareifying ASV reads for Alpha and Beta diversity analysis
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1
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62
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September 26, 2025
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Ancom-BC2 analysis along time series
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4
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154
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September 24, 2025
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Training a classifer for 18S and CO1 edna
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2
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95
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September 21, 2025
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Axis value percentages have no meaning in PCoA ?
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5
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160
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September 20, 2025
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Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis?
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4
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181
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September 18, 2025
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Percentage of reads discarded after running DADA2 on NextSeq data
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1
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71
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August 25, 2025
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Batch effects - Normalizing sequencing runs with different sequence counts
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3
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88
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August 20, 2025
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Interpreting usefulness of sequence data from quality scores
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3
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72
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August 15, 2025
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How to account for uneven sampling
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2
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91
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August 9, 2025
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Shall I need to remove ASV with prevelance in one sample?
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4
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109
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August 8, 2025
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Exclude significant results from betadisper from adonis2?
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0
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113
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July 19, 2025
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16S SRNA
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1
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87
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August 2, 2025
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Cage effect ANOVA
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11
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309
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July 31, 2025
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GSR database in QIIME2?
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2
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65
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July 28, 2025
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Problems with Taxonomic Assignment
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1
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49
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July 25, 2025
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Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)?
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4
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174
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July 22, 2025
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Sequence and taxonomy files for Silva v138.2
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2
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184
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July 21, 2025
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Beginner's Project
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2
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66
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July 21, 2025
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Recommended Step for Merging Metagenomics Datasets
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1
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82
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July 16, 2025
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Quality control for shotgun metagenomics
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5
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658
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July 17, 2025
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Merging qiime2 sample metadata with exterior taxonomic classifications
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1
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57
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July 13, 2025
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PR2: Division vs. Phylum
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1
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83
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July 11, 2025
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Is it necessary to separate ASVs classified at genus level into their respective sequences in ANCOMBC2 or other differential abundance analysis?
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2
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150
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July 8, 2025
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Transforming Alpha Diversity Metrics for Mixed Effects Modeling
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2
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68
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July 8, 2025
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Best workflow for samples with high off-target (host) amplification
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7
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159
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July 8, 2025
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High percentage of unassigned reads (metagenomics)
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6
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154
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July 3, 2025
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SILVA trained version for V3-V4 amplicon using 341F/785R primers
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3
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318
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July 2, 2025
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Looking for Sensible Qiime2 CLI Aliases
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1
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50
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July 1, 2025
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