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Closed reference OTU picking of V3-V4 amplicon against full-length reference database
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3
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72
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March 21, 2025
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Question about analyzing different sample types
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3
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99
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March 20, 2025
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SILVA vs PR2 database using qiime2
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1
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188
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March 19, 2025
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Contents of imported database
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6
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56
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March 18, 2025
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Missing species sequence on Greengenes2 2024.09
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2
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55
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March 18, 2025
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How important is it to remove spacers prior to DADA2 filtering trimming, etc?
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2
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50
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March 17, 2025
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Comparison of OTU Identifications with Silva 138.1 and Greengenes 2022.10 databases
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7
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201
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March 16, 2025
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ASV with missing taxonomic annotation
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2
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106
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March 11, 2025
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Dual-End Barcoding for 16S rRNA Primers: Using Golay Barcodes on Both Forward and Reverse Primers
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5
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70
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April 10, 2025
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How to download seven-number-summaries TSV directly
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1
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33
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March 7, 2025
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PCoA analysis Interpretation Doubt
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3
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59
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March 6, 2025
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Is it valid to rename ASVs classified as ‘uncultivated’ as ‘unclassified’?
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9
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121
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March 5, 2025
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OTU Tables Relative Frequency to Relative Abundance
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9
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164
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March 4, 2025
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how to phylogenetically analyse 16S genes recovered from metagenome?
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2
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73
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March 3, 2025
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Testing ITS1 Pipeline: Finding mock communities or correcting ITSxpress error
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7
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125
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March 3, 2025
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choosing of sampling depth
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7
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122
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February 28, 2025
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Animals gene family
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1
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33
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February 26, 2025
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tutorial for merging different sequencing data in qiime2
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6
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100
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February 26, 2025
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Sequencing bacterial 16S amplicons and Fungal ITS amplicons from same soil samples using same indices
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2
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84
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February 26, 2025
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Identification of Acanthamoeba in 18S rDNA analysis
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1
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31
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February 24, 2025
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Recommended timepoints for soil microbiome studies?
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5
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70
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February 19, 2025
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Code Review: Custom QIIME 2
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5
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58
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March 22, 2025
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Can MiSeq Reagent Kit v2 (500 cycles) be used to sequence V3-V4 region of bacterial 16S rRNA gene?
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4
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94
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February 19, 2025
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How to deal with multiple runs for one sample?
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8
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97
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February 18, 2025
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Nanopore Long Read Demultiplexed Full 16S rRNA Seq
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8
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1036
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February 17, 2025
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Issue with Taxonomy Assignment Using Kraken and Custom Database (BOLD, Nemabiome) in QIIME2
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4
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251
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February 14, 2025
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Uneven sample size, a need for rarefaction tool
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9
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147
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February 11, 2025
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Analysing merged sequences
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3
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63
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February 9, 2025
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non-fungal sequences in ITS2 analyses
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3
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109
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February 8, 2025
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Unnasigned ASVs at lower taxonomic ranks can alter metrics of alpha and beta diversity that use the phylogenetical distance?
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2
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55
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January 30, 2025
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