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Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)?
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4
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267
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July 22, 2025
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Sequence and taxonomy files for Silva v138.2
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2
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232
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July 21, 2025
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Beginner's Project
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2
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70
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July 21, 2025
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Recommended Step for Merging Metagenomics Datasets
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1
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93
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July 16, 2025
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Quality control for shotgun metagenomics
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2
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722
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March 13, 2024
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Merging qiime2 sample metadata with exterior taxonomic classifications
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1
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62
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July 13, 2025
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PR2: Division vs. Phylum
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1
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100
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July 11, 2025
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Is it necessary to separate ASVs classified at genus level into their respective sequences in ANCOMBC2 or other differential abundance analysis?
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2
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176
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July 8, 2025
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Transforming Alpha Diversity Metrics for Mixed Effects Modeling
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2
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77
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July 8, 2025
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Best workflow for samples with high off-target (host) amplification
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7
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169
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July 8, 2025
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High percentage of unassigned reads (metagenomics)
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6
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237
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July 3, 2025
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SILVA trained version for V3-V4 amplicon using 341F/785R primers
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3
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340
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July 2, 2025
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Looking for Sensible Qiime2 CLI Aliases
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1
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83
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July 1, 2025
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How to simplify the formula gini index in QIIME2? For example write in a R script.
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2
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75
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November 1, 2024
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Looking for Comprehensive Qiime2 Documentation
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3
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181
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June 23, 2025
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Request for Pre-trained SILVA V1–V3 Naive Bayes Classifier
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2
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104
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June 18, 2025
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classify-sklearn vs classify-consensus-vsearch - classification depth
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2
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167
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June 13, 2025
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Shotgun sequencing of Fungi - UNITE or KRAKEN2
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1
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131
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June 11, 2025
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Feature table and representative sequences for downstream analyses
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1
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48
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June 11, 2025
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PERMANOVA and DESeq2: Re-evaluating Covariates After Subsetting by Time Point
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1
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77
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June 9, 2025
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maarjAM database
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3
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220
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June 6, 2025
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Analysing with multiple public datasets
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4
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69
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June 5, 2025
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Qiime2 ConQuR Plugin for Batch Correction (or alternative)
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2
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175
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June 4, 2025
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Shotgun sequencing and Trimming
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2
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56
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June 3, 2025
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Can I run QIIME2 on de-multiplexed 5R 16S files?
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1
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73
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June 3, 2025
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Try to compare 16s and wgs with greengenes2
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0
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64
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May 16, 2025
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MAG filtering prior functional annotation
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2
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111
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May 28, 2025
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Using PCoA versus PCA
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6
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14284
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May 23, 2025
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Can trimming and dada 2
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3
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89
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May 19, 2025
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Knowing how many sequences did not get given taxonomy
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1
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31
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May 19, 2025
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