DADA2 priors trimming

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Hi Colin, i do have another question about prioring.

when I input a prior sequence i trimmed it down to v3-v4 region which is the one i used.
However is not fully clear if the search for priors is going to on the merged sequence, therefore leaving the 420bp length or does dada2 do it separately for fw and rev so i need to input priors trimmed down at the same length as truncLenF and truncLenR and separate them for fw and rev? or use only the fw?

thanks in advance for the attention

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Hello again Alberto! :waving_hand:

I'm sorry, I'm not sure I understand your question...

when I input a prior sequence...

This makes me think of the DADA2 error model is uses for error correction.

i trimmed it down to v3-v4 region which is the one i used

This makes me think of databases for taxonomy assignment.

Maybe you can share some of the code you have written?