Does QIIME2 support V1-V9 taxonomic classification?

General Discussion
1

Hi guys!

I wanted to create a 16S rRNA library of the V1 to the V9 regions. However, I wasn't completely sure that I could classify taxonomically those sequences after the library preparation. I have seen different questions about sequencing V1-V9 so I am guessing that it is possible but, is there any manuals of how to do it?

Thanks in advance

2

Hi @sgalera,

The short answer is, yes!

There are a couple of ways by which you can classify any region of your 16S rRNA gene library:

  1. Greengenes 2
  2. RESCRIPt
2 Likes
3

Thank you very much for your answer!