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High read loss after Deblur denoise-other despite permissive parameters and mostly uniform-length reads
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2
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54
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November 21, 2025
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Train PR2 Classifier
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4
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206
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November 20, 2025
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Observed ASV richness drops after adding 3rd MiSeq library — why do I lose ~100 species when I merge libraries?
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4
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37
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November 16, 2025
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ITS denoising truncation
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2
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60
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November 15, 2025
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Low % of reads pass merging and chimera filter after DADA2 for rSSU (Fungi)
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2
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110
|
November 15, 2025
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Interpretation of low "percentage of input non-chimeric" in DADA2 denoising stats
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2
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147
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November 15, 2025
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First-differences mis-handling of unequal time
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2
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55
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November 14, 2025
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Chimeras - dada2 settings, and optimal PCR polymerase?
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2
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35
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November 13, 2025
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ANCOM-BC analyis: comparing 3+ groups
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6
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140
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November 13, 2025
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Need help choosing truncation lengths for DADA2 and interpreting the demultiplexed sequence count summary.
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2
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48
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November 13, 2025
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training classifier SILVA: what is the best way?
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12
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300
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November 1, 2025
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quality-filter q-score Mínimum Threshold
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2
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50
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November 1, 2025
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How to obtain the 99% similarity clustering sequence of Greengenes2？
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2
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65
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October 21, 2025
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An error occurred when using Greengenes2
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6
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77
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October 20, 2025
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Importing Taxonomy into Qiime2
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8
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151
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October 19, 2025
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ABI reads with Ns: OTUs & classification
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4
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92
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October 14, 2025
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heatmap generation : clustered groups
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2
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47
|
October 13, 2025
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How to filter or reassign taxonomic names in QIIME 2?
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5
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79
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October 12, 2025
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Is ~25% final non-chimera read retention acceptable, and what is the expected range?
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2
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84
|
October 12, 2025
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How to build an OTU table using ONT sequencing
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6
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182
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October 4, 2025
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P value adonis test + ancombc
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4
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66
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September 27, 2025
|
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16S CCS without primers: how to skip --p-front/--p-adapter in denoise-ccs?
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4
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101
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September 26, 2025
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Effect of primer removal on chimera detection in DADA2
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11
|
208
|
September 26, 2025
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Question about consensus taxonomy assignment in classify-consensus-vsearch
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9
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194
|
September 23, 2025
|
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Is it valid to interpret rarefaction curves from DADA2 denoised feature table?
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2
|
82
|
September 19, 2025
|
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emperor barplot semicolon-delimited taxonomy
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2
|
29
|
September 11, 2025
|
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Best practices for detecting and removing outliers in beta diversity (after rarefaction)
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3
|
80
|
September 4, 2025
|
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Qiime2 NCBI adapters / primers filter
|
|
9
|
124
|
August 31, 2025
|
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HITDB classifier
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2
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35
|
August 30, 2025
|
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ancombc2 visualizer - da-barplor
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4
|
118
|
August 29, 2025
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