Hi,
To address this concern, I have been exploring potential solutions to improve the detectability and sequencing of the samples. One idea I am considering is the addition of known DNA sequences with sufficient concentrations that could act as "spike-ins" to enhance the overall DNA concentration in the sample. These spike-in sequences would ideally be detectable and distinguishable from my target DNA, allowing for more robust sequencing and subsequent downstream analysis.
I have a few questions regarding this approach:
Is it possible to add known DNA sequences to my samples as spike-ins to increase the overall DNA concentration for successful sequencing?
If so, what are the recommended types of known DNA sequences that could be added for this purpose?
What would be the best approach to quantify the added spike-in DNA and distinguish its sequencing data from the target DNA during downstream analysis?
Are there any potential challenges or considerations that I should be aware of when implementing this approach?
I would greatly appreciate your expertise and guidance on this matter. If there are other strategies you would recommend to address the issue of insufficient DNA concentration, I would be eager to learn about them as well.