Hi everyone, I would like to ask why the splicing rate between the left and right ends of my data is only 1%, and what are the possible reasons for this result? (Experimental and raw letter analysis) And how can we improve it?
It depends on the quality plots and region targeted. Usually I just go for forward reads. Note that using only one read (F or R) may lead to poorer taxonomy annotations, so I use it only when there is no other way to improve merging stats.