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PERMANOVA results significant, but differential abundance results detect 0 significantly different ASVs
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3
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142
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October 20, 2025
|
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Interpreting KEGG vs MetaCyc pathways in 16S (PICRUSt2) functional prediction
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1
|
69
|
October 18, 2025
|
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Rarefaction vs Rarefying and when to use them in your analysis pipeline
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16
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890
|
October 16, 2025
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Converting 16S V3–V4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?)
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3
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84
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October 9, 2025
|
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How to test for dissimilarity for my samples
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2
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270
|
October 9, 2025
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Rareifying ASV reads for Alpha and Beta diversity analysis
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1
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46
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September 26, 2025
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Ancom-BC2 analysis along time series
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4
|
91
|
September 24, 2025
|
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Training a classifer for 18S and CO1 edna
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2
|
63
|
September 21, 2025
|
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Axis value percentages have no meaning in PCoA ?
|
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5
|
101
|
September 20, 2025
|
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Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis?
|
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4
|
129
|
September 18, 2025
|
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Percentage of reads discarded after running DADA2 on NextSeq data
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1
|
59
|
August 25, 2025
|
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Batch effects - Normalizing sequencing runs with different sequence counts
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3
|
71
|
August 20, 2025
|
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Interpreting usefulness of sequence data from quality scores
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3
|
45
|
August 15, 2025
|
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How to account for uneven sampling
|
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2
|
64
|
August 9, 2025
|
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Shall I need to remove ASV with prevelance in one sample?
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4
|
81
|
August 8, 2025
|
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Exclude significant results from betadisper from adonis2?
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0
|
86
|
July 19, 2025
|
|
16S SRNA
|
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1
|
77
|
August 2, 2025
|
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Cage effect ANOVA
|
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11
|
282
|
July 31, 2025
|
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GSR database in QIIME2?
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2
|
45
|
July 28, 2025
|
|
Problems with Taxonomic Assignment
|
|
1
|
37
|
July 25, 2025
|
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Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)?
|
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4
|
130
|
July 22, 2025
|
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Sequence and taxonomy files for Silva v138.2
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|
2
|
126
|
July 21, 2025
|
|
Beginner's Project
|
|
2
|
55
|
July 21, 2025
|
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Recommended Step for Merging Metagenomics Datasets
|
|
1
|
66
|
July 16, 2025
|
|
Quality control for shotgun metagenomics
|
|
5
|
631
|
July 17, 2025
|
|
Merging qiime2 sample metadata with exterior taxonomic classifications
|
|
1
|
48
|
July 13, 2025
|
|
PR2: Division vs. Phylum
|
|
1
|
69
|
July 11, 2025
|
|
Is it necessary to separate ASVs classified at genus level into their respective sequences in ANCOMBC2 or other differential abundance analysis?
|
|
2
|
105
|
July 8, 2025
|
|
Transforming Alpha Diversity Metrics for Mixed Effects Modeling
|
|
2
|
54
|
July 8, 2025
|
|
Best workflow for samples with high off-target (host) amplification
|
|
7
|
113
|
July 8, 2025
|