Should I truncate all sequences to the same length for doing ITS?

Hi @Nicholas_Bokulich
Thanks for this information, I was not confident with the taxonomy results I got. Actually, I saw one very dominant group which is Fungi_Ascomycota in my feature table, which has a proportion of ~50%. It could only give me to phylum level but not any deeper. This is one of my concern. Is it normal? I don’t know how to lose the parameters to make taxonomy into deeper classification in this case.

Cheers