Hey everyone, I get my project report about a full-length 16S sequencing of 100 human fecal samples. And the company applied two different clustering methods (DADA2 and UPARSE) to get the ASVs or OTUs. But I found that the rarefaction curves of ASVs were totally different from those of OTUs, which mean that the same sample included the fairly low numbers of ASV but the fairly high numbers of OTU. Is that normal?
rarefaction curves of ASV
rarefaction curves of OTU