How to create taxa barplot using SRA toolkit on Mac

[Nicholas_Bokulich]

Hi @arundhati_brasilense ,
For getting data from SRA into QIIME 2, I recommend using the q2-fondue plugin for a streamlined process:

q2-fondue: Plugin for (meta)data retrieval from SRA

q2-fondue: (meta)data retrieval from SRA made simple Hello QIIMErs! On behalf of the Bokulich Lab team I would like to present to you our brand new plugin: q2-fondue (Functions for reproducibly Obtaining and Normalizing Data re-Used from Elsewhere)! q2-fondue allows you to grab any sequence data from the Sequence Read Archive (SRA) and corresponding metadata, given a list of SRA run accession IDs or BioProject IDs. Naturally, the resulting artifacts can then be directly plugged into …

@arundhati_brasilense

And how exactly can Qiime2 be used to generate the taxa barplot?

Detailed tutorials are available in the documentation at https://docs.qiime2.org/. After using q2-fondue to download data, it should already be demultiplexed and you can then follow any of the tutorials starting after the demultiplexing step (e.g., proceed directly to denoising).

Good luck!