Hi, everyone. I have some problems with qiime2 dada2 denoise-paired
The thing is that the same error is coming up.
I tried changing parameters (trunc, trim length) but same error is coming up.
qiime dada2 denoise-paired \
--i-demultiplexed-seqs merged_demux_seqs.qza
--p-trim-left-f 20
--p-trunc-len-f 290
--p-trim-left-r 0
--p-trunc-len-r 225
--p-max-ee-f 2
--p-max-ee-r 2
--p-chimera-method consensus
--o-table table_denoise.qza
--o-representative-sequences denoise-rep-seq.qza
--o-denoising-stats denoise-stats.qza
Traceback (most recent call last):
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/bin/qiime", line 11, in
sys.exit(qiime())
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 1137, in call
return self.main(*args, **kwargs)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 1061, in main
with self.make_context(prog_name, args, **extra) as ctx:
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 923, in make_context
self.parse_args(ctx, args)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/q2cli/click/command.py", line 43, in parse_args
return super().parse_args(ctx, args)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 1624, in parse_args
rest = super().parse_args(ctx, args)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 1375, in parse_args
parser = self.make_parser(ctx)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/q2cli/click/command.py", line 33, in make_parser
param.add_to_parser(parser, ctx)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 2658, in add_to_parser
parser.add_option(
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/q2cli/click/parser.py", line 126, in add_option
option = Q2Option(opts, dest, action=action, nargs=nargs,
TypeError: init() got multiple values for argument 'obj'
The thing is that I saw one of the forum topic that has same error (but related to rescript) so I followed (just in case), but exactly same error came out.
qiime dev refresh-cache
Traceback (most recent call last):
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/bin/qiime", line 11, in
sys.exit(qiime())
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 1137, in call
return self.main(*args, **kwargs)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 1061, in main
with self.make_context(prog_name, args, **extra) as ctx:
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 923, in make_context
self.parse_args(ctx, args)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/q2cli/click/command.py", line 43, in parse_args
return super().parse_args(ctx, args)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 1624, in parse_args
rest = super().parse_args(ctx, args)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 1375, in parse_args
parser = self.make_parser(ctx)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/q2cli/click/command.py", line 33, in make_parser
param.add_to_parser(parser, ctx)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 2658, in add_to_parser
parser.add_option(
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/q2cli/click/parser.py", line 126, in add_option
option = Q2Option(opts, dest, action=action, nargs=nargs,
TypeError: init() got multiple values for argument 'obj'
So, I thought the error was related to my qiime env so I removed and re-installed it for 4 times, but nothing has changed 
I really hope that the seniors who know this problem in the forum can help me,Thank you very much.