Hi, everyone. I'm new to qiime2.
I used qiime2 at one server, and I started DADA2 denoising step in the other server.
But, I am keep encountering same error. I thought it was something only related to DADA2, but as I searched in forum and tried all things I could do, It seems like the error is not confined to dada2, but whole environment.
First, I tried
(qiime2-2021.4) ~$ time qiime dada2 denoise-paired
--i-demultiplexed-seqs merged_demux_seqs.qza
--p-trim-left-f 20
--p-trunc-len-f 290
--p-trim-left-r 0
--p-trunc-len-r 225
--p-max-ee-f 2
--p-max-ee-r 2
--p-chimera-method consensus
--o-table table_denoise.qza
--o-representative-sequences denoise-rep-seq.qza
--o-denoising-stats denoise-stats.qza
Traceback (most recent call last):
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/bin/qiime", line 11, in
sys.exit(qiime())
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 1137, in call
return self.main(*args, **kwargs)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 1061, in main
with self.make_context(prog_name, args, **extra) as ctx:
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 923, in make_context
self.parse_args(ctx, args)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/q2cli/click/command.py", line 43, in parse_args
return super().parse_args(ctx, args)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 1624, in parse_args
rest = super().parse_args(ctx, args)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 1375, in parse_args
parser = self.make_parser(ctx)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/q2cli/click/command.py", line 33, in make_parser
param.add_to_parser(parser, ctx)
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/click/core.py", line 2658, in add_to_parser
parser.add_option(
File "/data1/hjpyun/.conda/envs/qiime2-2021.4/lib/python3.8/site-packages/q2cli/click/parser.py", line 126, in add_option
option = Q2Option(opts, dest, action=action, nargs=nargs,
TypeError: init() got multiple values for argument 'obj'
And even if I type qiime --help, same error comes up
So I even re-installed qiime for 4 times with new conda env, but didn't work...
What should I do..?
Thank you!
