Hello everybody I’m looking for a way to make a closed-reference otu from a fastq files from single reads that are already fully processed (both barcodes and primers was removed and quality filtering performed). Is this possible? I read that this is possible with q2-vsearch in QIIME2 but I tried without any successful…
How many files do you have? Typically we have multiple fastq files (one or two per sample). I think the issue is you are using the SampleData[Sequence] type which is for sequences that don’t have quality information. You probably want SampleData[SequencesWithQuality] and one of those corresponding formats (which I can assist with once I know more).