Hello everybody I’m looking for a way to make a closed-reference otu from a fastq files from single reads that are already fully processed (both barcodes and primers was removed and quality filtering performed). Is this possible? I read that this is possible with q2-vsearch in QIIME2 but I tried without any successful…
qiime tools import \
There was a problem importing head.fastq:
head.fastq is not a(n) QIIME1DemuxFormat file
can anybody give some suggestion please?
Thanks a lot!