I am currently trying to analyze data from 16S amplicon sequencing (Nextera XT chemistry for Illumina), and during sequence processing with DADA2, I am getting only a small percentage (~ 50%) of nonchimeric sequences.
I read in previous posts that this can be resolved by tuning the "--p-min-fold-parent-over-abundance" parameter. My question is, how to decide what is the right value that fits my dataset?
Attached you can find the qzv file with the quality of my reads, and the descriptive statistics of the results.
Thank you in advance!