Unable to obtain taxonomy using blast!

Shriya_Sawant · July 25, 2020, 6:29am

I have been trying to classify my sequences using consensus-blast feature, but am unable to do so. Can someone let me know what could be the issue ?
Thankyou.

Nicholas_Bokulich · July 29, 2020, 2:13am

Hi @Shriya_Sawant,
SIGKILL 9 usually means that the job was terminated because you ran out of memory. blastn does not usually require too much memory, and you only have a few options:

run on a higher-memory machine, or allocate/request more memory (e.g., if using a virtual machine or compute cluster)
use a smaller reference database (e.g., try greengenes instead of silva)

I hope that helps!

Shriya_Sawant · July 29, 2020, 4:34am

Thankyou very much @Nicholas_Bokulich for your reply. I will definitely look into these points and hopefully should get through. Thankyou!