Hi there
I want to use the Silva 138 data base in QIIME1 for my analysis. I have done most of the statistical analysis of my manuscript by using data obtained from QIIME1. Considering the comment of the reviewer, I want to assign taxonomy using Silva 138.
Will you please help me to train the silva 138 data base for use in QIIME1?
I need basically consensus_taxonomy_7_levels.txt and silva_138_99_16S_515-806.fna files.
I will be very grateful for this help.
Thank you in advance
Rajendra
As of 1 January 2018, QIIME 2 has succeeded QIIME 1.
QIIME 1 is no longer officially supported and all development and support efforts are now focused entirely on QIIME 2.
That said, it should still be possible to do your taxonomic classification with QIIME 2. You can import your QIIME 1 representative sequences file to QIIME 2 (see "importing" tutorials at the website below), classify with one of the pre-trained SILVA classifiers (see the website below), and then export the data for further analysis (even in QIIME 1 if you wish).
You can refer to https://qiime2.org to learn how to install and start using QIIME 2.
If you have any specific questions about QIIME 2, please open a new topic.
Good luck!