Problem with Biom add metadata

[YuZhang]

Exporting and modifying BIOM tables (e.g. adding taxonomy annotations)

Creating a TSV BIOM table The following is based on a post by @gregcaporaso. $ qiime tools export table.qza --output-dir ./ $ biom convert -i feature-table.biom -o feature-table.tsv --to-tsv $ biom head -i feature-table.tsv # Constructed from biom file #OTU ID L1S105 L1S140 L1S208 L1S257 L1S281 4b5eeb300368260019c1fbc7a3c718fc 2222.0 0.0 0.0 0.0 0.0 fe30ff0f71a38a39cf1717ec2be3a2fc 5.0 0.0 0.0 0.0 0.0 d29fe3c70564fc0f69f2c03e0d1e5561 0.0 0.0 0.0 0.0 0.0 868528ca947bc57b69ffdf83e6b73bae 0.0 227…

I did it like according to above,and my command like below

  qiime tools export \
      --input-path /share/disk0/user/maj/YuZhang/micro_plastic_2020/16s/Download/table-dada2-3.qza \
      --output-path exported-feature-table \
    qiime tools export \
      --input-path /share/disk0/user/maj/YuZhang/micro_plastic_2020/16s/Analysis/try1/taxonomy.qza \
      --output-path exported-taxonomy-table \
      cp exported-taxonomy-table/taxonomy.tsv biom-taxonomy.tsv
      biom add-metadata -i exported-feature-table/feature-table.biom -o table-with-taxonomy.biom --observation-metadata-fp biom-taxonomy.tsv --sc-separated taxonomy
      biom convert -i table-with-taxonomy.biom \
        -o table-with-taxonomy.txt \
        --to-tsv 

and I changed the first line in biom taxonomy,as follow:

but ,the result is

There isnt taxonomy.I dont know why.
Please help me.

Yu

[YuZhang]

Sir，I used the measure you shared，but it didnt work.

biom add-metadata -i exported-feature-table/feature-table.biom -o table-with-taxonomy.biom --observation-metadata-fp biom-taxonomy.tsv --sc-separated taxonomy
biom convert -i table-with-taxonomy.biom
-o table-with-taxonomy.txt
--to-tsv