Hello,
I made the V3-V4 region classifier, as was mentioned before in the forum, with these primers:
341F: CCTACGGGNGGCWGCAG
805R: GACTACHVGGGTATCTAATCC
According to Illumina’s 16S library protocol (2*300 bp).
I followed the steps in the tutorial and used this options in the Extract reference reads section:
qiime feature-classifier extract-reads
--i-sequences 99_otus.qza
--p-f-primer CCTACGGGNGGCWGCAG
--p-r-primer GACTACHVGGGTATCTAATCC
--p-min-length 100
--p-max-length 600
--o-reads ref-seq.qza
I used qiime2-2021.11 (scikit-learn (0.24.1)). gg13_8
I'm pretty new to QIIME, so please let me know if I made a mistake in preparing the file.
Here is the file:
Pre-trained Classifier of V3-V4 region with gg-99
Thanks!