I made the V3-V4 region classifier, as was mentioned before in the forum, with these primers:
According to Illumina’s 16S library protocol (2*300 bp).
I followed the steps in the tutorial and used this options in the Extract reference reads section:
qiime feature-classifier extract-reads
I used qiime2-2021.11 (scikit-learn (0.24.1)). gg13_8
I'm pretty new to QIIME, so please let me know if I made a mistake in preparing the file.
Here is the file:
Pre-trained Classifier of V3-V4 region with gg-99