Unfortunately, I ran into another snag. Trying to use the quality filtering, deblur, and vsearch commands resulted in an error concerning whitespace in my file. To debug this, I validated my demux.qza file and received the following message:
Artifact demux.qza does not appear to be valid at level=max:
/tmp/qiime2-archive-1mrp3icd/f913f599-1179-4b98-be30-ce6fddc9d83e/data/Sample67_22_L001_R1_001.fastq.gz is not a(n) FastqGzFormat file:
Missing sequence for record beginning on line 5157
Clearly, my artifact file isn’t valid. Is there any method to correct this?