Hello again, everyone, I'm posting this in hopes of getting more help running Dada2. I solved my memory issue by gaining access to a local server with more computing power than my current PC, but now I'm encountering a new issue. After running for about a week and a half, this error presented itsel…

Hi there @Brightbeard ! [image] Brightbeard: [Errno 2] No such file or directory: ‘/tmp/tmpdftfsbgd’ This looks like your host OS might've cleaned up your temp dir before the job had finished processing. First off, it looks like you aren't trimming or truncating your reads at all --- this ca…

First off, it looks like you aren’t trimming or truncating your reads at all — this can have a huge impact on DADA2 runtime — it is a function of the quality of the reads, so by trimming low quality positions, you help reduce the burden applied to the algorithm. If you would like us to weight on on…

Thanks for sharing that viz, @Brightbeard ! Here is a screenshot of the quality plot in that viz: [41%20PM] Two things jump out at me: These reads appear to be pre-joined. Can you confirm that? It is worth noting that DADA2 expects to operate on unjoined reads (this is because the algorithm uses…

These reads appear to be pre-joined. Can you confirm that? They are. My lab is currently in transition from our old 454-pyrosequencing primers to Illumina primers, as we are waiting on grant funding to purchase new Illumina primers. This means I have to do some work in Qiime1 first in order to or…

Thanks for the info, @Brightbeard . Unfortunately, as I understand it, you probably shouldn't send these reads through DADA2, since it violates a few assumptions (note, maybe in the future we will get a q2 plugin for unifying read orientation). Check out the second half of this tutorial for a deblur…

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Dada2 [Errno 2] No such file or directory: '/tmp/tmpdftfsbgd'

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