The sequencing facility told me that their ITS2 primers are proprietary, so they cannot provide the sequences, but only the length of the primers. But, the sequences of the primers are needed to trim out the read-through to the opposite primers according to the ITS workflow (Fungal ITS analysis tutorial and https://benjjneb.github.io/dada2/ITS_workflow.html)
Is there any possibility to check if there are read-throughs and trim them out with only the length of the primers?
Thank you