Import --i-reference-taxonomy taxonomy.tsv to .qza

[jairideout (Jai Ram Rideout)]

@Bing_Guo

did try DADA2 with one sample (84,000 reads in either forward or reverse), after 5 hours, not finished. So I ctrl+c the job. I'm using a MacBook Air, which is not a powerful computation laptop.

Did you use DADA2 in multi-threaded mode by passing --p-n-threads? If so, how many threads did you specify?

It's possible your job either ran out of memory, or too many threads were specified with --p-n-threads. If you end up rerunning DADA2 on your MacBook Air or on the Amazon cloud, I recommend monitoring both memory and CPU usage while the job is running to see if the system is getting overloaded.

You could also try running DADA2 on a very small subset of your sequence data, just to confirm that it can complete successfully on your computer.

@Bing_Guo

Also, our lab is planning to invest on a new desktop for the qiime2 computation. What could be your suggestions on the hardware, RAM, storage, etc?

This question has come up a couple of times before, see these forum topics:

QIIME 2 native install on Macbook?

Hi! I was wondering if anyone had any experience or recommendations natively installing and running QIIME 2 on a laptop (specifically MacBook Air with Intel i5 processor, 8GB of RAM, and 128 GB storage). Currently I have QIIME 2 natively installed on an iMac in my office at university. I am thinking of buying a new laptop and thought it might be useful to be able to run QIIME analyses away from the office (probably not demultiplexing or denoising steps, but things like diversity analysis). I h…

QIIME 2 Native installation

Hi, We have always used QIIME virtual box, and recently we have been thinking about investing in a machine where we could natively install QIIME2. We would ideally like the transition to be as easy as possible and needed some suggestions on what platforms are recommended. I read that it can be installed on Unix/Mac platforms, would one be better over the other?