I have tried Calypso with different methods, so let me inform you about how far i could go.
First time, I tried Calypso with NCBI taxonomy file. It didn’t parse. Something like unassigned taxa was the result.
Then, I suspected if it was configured to work with some other database since i could get results with ncbi taxonomy file (like taxa bar plots were looking like what i expected) but Calypso didnt work. So i tried it with greengenes database and this time it seems like it worked but i have problems.
Problem one is it doesnt show level 7 (species). There are seven layers, seven levels but while parsing the data it added something before level 1 (kingdom) and species are lost.
Problem two is i had eleven samples. Krona doesnt show them seperately, i guess it got all of them summed up. Is there a way to make it work for each sample seperately?
This is the link to the Krona plot i got. Hope someone could help me because i really want an interactive plot like Krona.