I am running into some weird questions when I am doing fungi taxonomy work with Illumina result on Qiime 2.
Fungi gene were sequenced on ITS1and 2 primer on Illumina platform. The result came back like this.
I thought it could be a wrong choice for ITS gene database (I used SILVA full dataset include ITS genes and matched to UNITE taxonomy)
But when I decided to extract all the entries and relative frequencies of each entry, the abundance does not match.
This is ranked from highest abundance to lowest.
Apparently, the first entry (~25%) does not match the high abundance from the taxonomy (~50%).
I BLASTed each single entried and it returns with mostly Kazachstania sp.
I am having a hard time figuring out where I had done wrong.
Could Qiime combine entries it cannot classify into one big entry and show in the taxonomy bar plot?
What can I do to trouble shoot?
Thanks a lot!
If anything does not make sense, I can add more information on!