Extract reads - SILVA 128 issue

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Hi @Azra_Music,
It looks like you are using the 515f/806r primer pair for 16S rRNA gene sequences. Why not just use the pretrained classifiers for SILVA or Greengenes that are available on the QIIME 2 website?

Does this post or this post describe your issue? Those sound like what you describe — the tips in those posts may help resolve your issue.

If those posts do not help resolve this issue, please give us some more details:

  1. What read length do you have?
  2. single-end or paired-end reads?
  3. What sequencing protocol are you using?

Thanks!

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