I am a beginner in Qiime2 so I need your help.
I am trying to complete official Qiime2 tutorials and I have a problem about " Parkinson’s Mouse Tutorial (https://docs.qiime2.org/2020.8/tutorials/pd-mice/#parkinson-s-mouse-tutorial)".
The problem occurs at the step of “Generating a phylogenetic tree for diversity analysis”.
When I write this codes:
qiime fragment-insertion sepp
–i-representative-sequences ./dada2_rep_set.qza
–i-reference-database sepp-refs-gg-13-8.qza
–o-tree ./tree.qza
–o-placements ./tree_placements.qza
–p-threads 1
I get this error:
Plugin error from fragment-insertion:
Command ‘[‘run-sepp.sh’, ‘/tmp/qiime2-archive-xl4ysncq/fcec470e-99cb-43e7-835c-1a98e9ba400e/data/dna-sequences.fasta’, ‘q2-fragment-insertion’, ‘-x’, ‘1’, ‘-A’, ‘1000’, ‘-P’, ‘5000’, ‘-a’, ‘/tmp/qiime2-archive-tc2dafpm/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/aligned-dna-sequences.fasta’, ‘-t’, ‘/tmp/qiime2-archive-tc2dafpm/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/tree.nwk’, ‘-r’, ‘/tmp/qiime2-archive-tc2dafpm/a14c6180-506b-4ecb-bacb-9cb30bc3044b/data/raxml-info.txt’, ‘-b’, ‘1’]’ returned non-zero exit status 1.
I started from the beginning multiple times but the error has not changed yet. I have searched on the internet but could not find any solution for.
I have downloaded Ubuntu from windows market on my windows 10 laptop which has 4 GB ram. Can the low amount of ram be the problem?
As a beginner, I would appreciate it if you could explain it to me clearly.
Thank you for your interest!