problems with qiime fragment-insertion sepp

[SoilRotifer (Mike Robeson)]

Okay, just wanted to sanity-check. Here here are other forum posts on this issue. I suggest reading through these:

Plugin error from fragment-insertion - CalledProcessError - run-sepp.sh - non-zero exit status 1

Hello, I am trying to work on Picrust 2 via the QIIME2 plugin, but I am stuck on the first step which uses the plugin q2-fragment insertion. I have looked through the forums but cannot not seem to find a definitive answer. I was hoping someone could help me out! Disclaimer: I'm using QIIME2-2018.8, running via Ubuntu for Windows10. We successfully used QIIME2-2018.6 using this method. But we wanted to work with picrust2 and there was also an update to QIIME2 so it happened all together - hope…

Fragment-insertion sepp returned non-zero exit status

Hi @Nicholas_Bokulich thank you! To try and narrow down the error I ran the following command using the default greengenes database: qiime fragment-insertion sepp \ --i-representative-sequences rep-seqs.qza \ --o-tree insertion-tree.qza \ --o-placements insertion-placements.qza And it seems to be working so I think the issue is my reference alignment and phylogeny rooted_tree.qza (367.0 KB) alignment.qza (2.5 MB) Thanks for your help!

Error from Fragment-Insertion Sepp - non-zero exit status 1

I am a beginner in Qiime2 so I need your help. I am trying to complete official Qiime2 tutorials and I have a problem about " Parkinson’s Mouse Tutorial (https://docs.qiime2.org/2020.8/tutorials/pd-mice/#parkinson-s-mouse-tutorial)". The problem occurs at the step of "Generating a phylogenetic tree for diversity analysis". When I write this codes: qiime fragment-insertion sepp --i-representative-sequences ./dada2_rep_set.qza --i-reference-database sepp-refs-gg-13-8.qza --o-tree ./tree.…