MiSeq_data_v3_table_min_8_feature_filtered_table.qzv (739.7 KB) MiSeq_data_v3_table_min_8_feature_filtered_table.qza (489.1 KB) Hi User I am constantly getting an error while performing qiime gneiss ols-regression. "Detected zero variance balances - double check your table for unobserved features…

Hey there @dheeman00 - just wanted to let you know we are looking into this issue right now, but it might be a few days before you hear back from someone. Stay tuned! :t_rex:

@dheeman00 , looks like you will need to filter out those zero-variance features. I'd take a look at the qiime feature-table filter-features command There are a couple of posts that discuss this problem in more detail [image] Integer division or modulo by zero Error User Support …

@thermokarst Thank you for looking into the issue. Just a query. Is it a sort of a flaws in the qiime 2 platform.

Hi There Thank you for your post. In the second suggestion you have mentioned that there some values of singleton and doubleton. Is it possible to know how I can identify them form my dataset. I could not upload the balance file as it is bigger than the file uploading limit. But some suggestions wi…

Hi @thermokarst and @mortonjt Thank you for your suggestions. I was able to resolve the issues. I figured out the issue was not that complicated just proper explanation was missed out, which you guys have provided.

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Detected zero variance balances while performing "gneiss ols-regression"

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feature-classifier, taxonomy, filtering, metadata, feature-table

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