Data is demultiplexed or not? And DADA2 or Qiime2 as a pipeline?

Hi @EnyaroHatsonveski ,

As you are re-using data from SRA, it might be easier to use q2-fondue, which will download, reformat, and import to QIIME 2 in a single step. See this tutorial (and please open a new topic if you run into issues using q2-fondue):

But otherwise you can use the "Manifest" format (see the QIIME 2 online documentation for a tutorial) to import the data that you have already downloaded.

Good luck!

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