CZI-CABANA Workshop discussion thread

Hi @the_dummy,

I agree on fastqc. To answer your question, not necessarily. It may depend on the prep kit and where are sequencing adapters. In my experience, the length of 250 bp includes everything, sequencing adapters (depending on kit ), barcodes as well as PCR primers and low-quality tails. Hence, for me when I saw 250 bp it means these are as raw as possible (for a 2x250 bp MiSeq run at least)

Cheers

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5 posts were split to a new topic: Why not use 100% database identity?

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Hi @llenzi,

In that case, I’m mistaken to rely only on my experiences.

Thank you very much!

4 posts were split to a new topic: sequence length variation after dada2

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Friends,

A general, gentle reminder that this thread is not actively monitored during the workshop and you will probably have more success getting your technical and general questions answered if you post it in a unique thread.
I encourage you to use “General Discussion” for theory, “User Support” for specific issues, and “Technical Support” for bugs.

Thanks for your interest and participation!

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Hi,
Warm greetings!
Thank you for your response and suggestion. I will keep that in mind next time I post a question. Am curiously waiting for some answers too.
That was very helpful.
Thank you very much.
My sincere regards.

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