CZI-CABANA Workshop discussion thread

A post was split to a new topic: Are there conflicts between QIIME 2 Installed in VirtualBox and the host machine?

Hi guys,
thanks for that amazing first day! Unfortunately I have no access to the livestream video anymore… Will u upload parts of the workshop on your youtube channel?

Hello all thanks for setting up the workshop and trying to accommodate everyone. I know you plan to only have the livestream video during the workshop, but the video is currently set to private. I was behind the stream by a couple hours and it ended suddenly and I have no way of continuing and following along.

Please consider making the video available during this week at least. The messy registration screwed over a lot of people interested!!

I’d also second (third) the suggestion for YT chat, only because it is live/on the same page as the video and would be more immediate feedback between streamers than using the forum. The community could police each other while you all focus on the workshop and Slack.


Hi @rinne_sharingan! Yes, we are planning on uploading all of this, some time in the first 2 or 3 months of 2021 - watch the forum for more details!

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You’re welcome!

We are planning on uploading all of this, some time in the first 2 or 3 months of 2021 - watch the forum for more details.

We can’t support that, but you are welcome to set up and support a chat option on the service you prefer. As always, we are happy to answer questions here on the QIIME 2 Forum.

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Alright, on that note, any additional questions that come in on this topic thread will not be answered, unless they represent completely new questions that haven't been asked and answered before. We have answered the same 2 or 3 questions many, many times at this point, and we would like to be able to continue to provide support to others here on the QIIME 2 forum, asking other questions outside of this topic thread. If you post a question and don't hear from anyone, then take that as a sign that the answer you seek lies up above in the discussion.

Thanks for your understanding!



Hi @ChrisKeefe,

Wondering whether you could make the livestream video until the next day workshop?

Due to the time difference (>12 hours), people from the Asian region, such as China, Singapore, Japan, etc. actually cannot attend the full course. If people could watch the video before the next day workshop, that would be fantastic and people can still catch-up.

Hi @thermokarst, thanks a lot for your kindness and willingness to upload videos. That’s really fantastic. Just wondering whether it is doable to make the livestream video available until the next day’s workshop, so people from Asian region can still catch-up.


The time difference is really a problem. Please if possible make the video available for the next day at least. It is very difficult to catch up in Australia. I tried last night but it was very late.


It will be highly appreciated if it is possible to share the recording of yesterday’s session before today’s session since I had missed out the metadata segment of the session due to the difference in our time zones.

I am requesting this since I am a beginner in this area and it will be very difficult for me to follow the next segment.

I had tried for registration but seats were unavailable and hence I had opted for the free-viewing option.


This livestream url doesnt exist anymore. Is there any way how we can refer to the videos post live session?


Hi, will the youtube kink for today program be given

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If you start the livestream and then pause it - it can be paused for any length of time and you can come back and watch it (ie the next day if time zones are an issue). I tested this last night and even with the stream hiccups the ‘unpaused live’ stream was flawless. If you do not start it when the stream begins, I do not think it is possible to go back and access it. Hope this helps


Today’s Live Stream can be found here:

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Same for me, the YouTube link is showing as restricted today when yesterday I was able to view it just fine.

Maybe you try searching “QIIME 2” on YouTube

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The video is now working but the audio is shut down, can you guys listen to it?

Thanks for this chance!

yes i can listen to it, very smoothly

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Hello @chiara ! I am currently listening to the youtube stream and there is audio. I would suggest checking your personal set up. Hope this helps!

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To add to the discussion about raw sequences and what is inside;

I think you can check the length of the sequences to see if it is longer than the expected size. Let’s say read length is 2x250. Then, if we have sequences longer than 250 bp, doesn’t it mean that there are barcodes or index or something else?

Also, an option out of Q2, FastQC should show if there are Illumina barcodes in fastq files if I’m remembering correctly.

Lastly, yes, there is not a specific way to tell what is inside our files if it is not delivered with right info.

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