create NCBI database

Hey,
I’d like to use NCBI database for classification. I can find taxonomy/sequences for Silva and GreenGenes, but there’s no any hints how to create such NCBI-based database.

Any suggestion how to perform it? Should I download all bacteria sequences one-by-one or there are already some tricks how to do it fast?

Thanks

1 Like

Hi Damian, hope all is well, there’s some threads on this:

Personally, I haven’t used these methods, but briefly looking through these archived threads (and one more recently), it’s not different than using the 16S gene databases (which I am most familiar with greengenes). It looks like you will have to do some work to get it working. Ben

1 Like

Great, thanks, I’ll have a look on that!.
I don’t want to use GreenGeenes as it’s 6 years old now ;(

1 Like

Yeah, the lab still uses greengenes as some of the inferred metagenome is connected to greengenes annotations. Ben