Can someone tell me how to use brocc, please

I'm using brocc to return the following, but I'm using test data that is clearly provided on the official website, and again I'm returning the same error with my own data。
Error:
Plugin error from brocc:
Command '['blastn', '-query', '/tmp/qiime2/xiaoe/data/50c33ae5-dfe9-4b83-a607-8579e0a27090/data/dna-sequences.fasta', '-evalue', '1e-05', '-outfmt', '7', '-db', 'nt', '-max_target_seqs', '100', '-out', '/tmp/tmp14oivpy7']' returned non-zero exit status 2.

Debug info has been saved to /tmp/qiime2-q2cli-err-9vexhi0r.log

@wupengjie,

Unfortunately the bare brocc tool is not available in the QIIME 2 ecosystem and we are not able to offer support for i, hence why this thread was locked earlier. I am not sure if its developers offer any kind of support. I will move this thread to our "other bioinformatics tools" section and maybe some other users will be able to help you out! I would advocate for performing classification using QIIME 2 with the tools available in the q2-feature-classifier plugin :slightly_smiling_face:

2 Likes