I’m trying different strategies in clustering features in my dataset: closed, open reference and de-novo.
I previously imported and created the required taxonomy.qza file starting from the latest version of GreenGenes database.
I filtered chimeras and then wanted to create a barplot for each of the clustering, to compare results and perform taxonomic analysis.
Everything worked fine for closed-reference analysis, but with denovo and open-reference ones it didn’t. In the case of de novo one, I used the following code:
qiime taxa barplot --i-table uchime-dn-out_cr_new/table-nonchimeric-wo-borderline.qza --i-taxonomy gg_13_8/gg_13_8_otus/taxonomy.qza --m-metadata-file metadata.tsv --o-visualization taxa-bar-plots_cr_concloroplasto.qzv
And this is what I got: ValueError: Feature IDs found in the table are missing from the taxonomy.
Here is the whole log.
I think I’m missing something very basic here, can anyone help? It’s an open reference/de novo analysis but the script still asks me for some taxonomy file?
Should I use a different one?
Thanks in advance!
I’m running QIIME2 2019.7