Hi everyone
I found this topic and felt that you could help me regarding the quality of my data.
I have sequences from am Ilumina Miseq run (Using a 16s analysis in the V3-V4 region), I receive the sequences demultiplexed and import them in qiime2. So I got the demux.qzv file and put in the qiime view, and this is my result:
So I'm confused where I have to truncate and trim? The middle of my sequences are in a too low quality, this is unusual because normally we see the low quality in the final of the sequences, how can I deal with it?
Thanks in advance