There are two ways to create otu table-using the table after “denoise-singe” or “vsearch cluster-features-de-novo” function. I am confusing which way is better to be applied to further analysis. Does anyone have good suggestions?
There are good reasons to cluster your data after denoising and good reasons to leave it alone. I think you would be interested in this recent topic, which covers a lot of ground related to your question: