Which table (after denoiseing or clustering) is better to be used to create otu table?

There are two ways to create otu table-using the table after “denoise-singe” or “vsearch cluster-features-de-novo” function. I am confusing which way is better to be applied to further analysis. Does anyone have good suggestions?

Hi @kaile!

There are good reasons to cluster your data after denoising and good reasons to leave it alone. I think you would be interested in this recent topic, which covers a lot of ground related to your question:

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Thanks so much for your clear clarification!