Which position is best for trimming in a paired-end approach?

So I've checked the mean length of my reads here:

Great!

Next, how long is the amplicon you sequenced?

Then calculate the overhead as shown here!

It is common for reverse reads to show a decrease in the quality of the reads?

Yes, though it's usually not this bad. Take a look at this example from the Atacama soils tutorial.

Because my last two sequencings my analyses were conducted by a single-end approach, due to bad quality of the reverse reads.

Maybe a problem with the PCR, or the sequencing core, or something spooky! :ghost: